báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx

... article The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathwayCinzia Comino1, Alain Hehn2, Andrea Moglia1, Barbara Menin1, Frédéric ... GGGTTTCATATGACTATCGGAGCTCGTGATHQT-Rev CGGGATCCCTAGAAGTCATACAAGCATTTHCT-ForRT TTTTTAAGCTAACACGAGACHCT-RevRT TCTCATAGGAGCTGTAATTGHQT-ForRT TAAAATGGACGATCAGTATCHQT-RevRT TTATGTTCAGATTTGGACTCACT-ForRT TACTTTCTACAACGAGCTTCACT-RevRT ... CACGTCGGCTTCGACTGTAGGTCGACTHCT-OuterFor CACGAGACCAAGTCAATGCACTCAAAGGAHCT-OuterRev GATTCGGGCACTTAAACGTATGAGCCCCHQT-InnerFor CGTGGACTATCAGACGATCAACCATCCHQT-InnerRev TCGTCCGTCAGTAGCCACGTACAGTATCHQT-OuterFor CACAAAACCAAAACTTCACATCCCATCCHQT-OuterRev...

Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... reactions. The ﬁrst round of PCRreaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGCAAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAATACGACTCACTATAGGGC-3¢) and ... PCRreaction was performed using a pair of speciﬁc primersP3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4(5¢-ATAACAACAGTTTATACATCGCTTC-3¢) correspon-ding to the 5¢-untranslated and 3¢-untranslated ... Comparison of the amino- acid composition of the calcium-binding domains in canonical EF-hands [30] with that in the EF-hands in oyster CaLP, reveals a good correlation of Ca2+-bind-ing ligand...

Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx

... 1297Cloning, characterization and localization of a novel basicperoxidase gene from Catharanthus roseusSantosh Kumar, Ajaswrata Dutta, Alok K. Sinha and Jayanti SenNational Centre for Plant Genome ... Skoog (MS)3basal medium by painting on the adaxial surface of the leaves, and the tray containing the leaves was sealed with saran wrap. In control experi-ments, similar leaves were painted with ... properties. Two of these leaf-speciﬁc dimeric alkaloids, vinblastine and vincristine,are used as valuable drugs in cancer chemotherapy.Owing to the medicinal importance of these alkaloids and their...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... lectin on SDS/PAGE gave a singleband at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater crab, Paratelphusa jacquemontii, expressedO-acetyl ... showing a( 2–6) linkage. The sialic acid affinity of the Paratelphusa lectin wasfurther proved by its inability to inhibit sialidase-treatedBSM and bovine thyroglobulin. Also the lectin failed ... in identifying and discriminating sialic acids on the surface of cancer cells. The sialic acid- specific lectins are also useful in distinguishing highly pathogenic strains of bacteria [15–19]. Of the...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... behavior istypical of integral membrane proteins. The polypeptide with anapparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1).Table 1. N-Terminal ... Materials and methods. (A) Data pointscorrespondtotheamplitudeofthetroughcenteredatg ¼ 1.951; as in the low po tential range, the radical signal of the dyes overlap in the g ¼ 2.0 region. The maximal ... beendeposited in the databases. None of these putative pro teinshas b een c haracterized and no f unction has been assigned toany of them [2]. In this study, we chose the sulfate-reducingarchaeon A. ...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... binding of SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the ... of A DNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal extension. (B) Alignment of ligandbinding domain ... proceduresParasites The NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian golden hamsters(Mesocricetus auratus ). Cercariae were released from infec-Fig. 11. DNA binding...

Báo cáo khoa học: Identiﬁcation and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

... pull-down and immunoblotting against anti-HA serum. Coomassie Brilliant Blue staining and anti-HA blotting of the input materials ascertain the equivalentamounts of extracts and peptides between each ... transcriptionalactivation (amino acids 1–63), N-terminal Elf-1(amino acids 63–244), the basic (amino acids 244–250), SPXX (amino acids 250–403), Q ⁄ P (amino acids403–413) and C-terminal acidic (amino acids 413–502)regions ... Sugihara TM, Wang N, Lasso RJ,Gudnason JF, Lipkin SM & Andersen B (2003) Identiﬁ-cation and characterization of Grainyhead-like epithelialtransactivator (GET-1), a novel mammalian Grainy-head-like...

Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

... GSTs in insects [2]. The majority of GSTsare in the Delta and Epsilon classes, and the remain-ing enzymes are in the Omega, Sigma, Theta and Zetaclasses. The German cockroach (Blattella germanica)isaneconomically ... PointRichmond, CA, USA).Amino acid sequencingEdman degradation, performed by Proteos, Inc. (Kalama-zoo, MI, USA), was used to determine the amino acid sequence of 20 N-terminal residues. The amino acid sequence ... obtained by Edman degradation and LC ⁄ MS ⁄ MS analysis of the puriﬁed BgGSTD1 werecrucial for the isolation of cDNA. The N-terminalamino acid sequence provided essential information toindicate...

Báo cáo khoa học: Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis pdf

... 2).Activation of class I PI3K inhibits autophagythrough activation of protein kinase B (Akt) and mammalian target of rapamycin (mTOR). In contrast,activation of class III PI3K in a complex with the autophagy-associated ... 1-depen-dent autophagy in yeast and mammalian cells [4].Beclin 1, the mammalian ortholog of yeastAtg6 ⁄ Vps30, was originally discovered in a yeast two-hybrid screen as a Bcl-2-interacting protein, and ... betweenautophagy and apoptosis by Bcl-2 and Bcl-xLMany signaling pathways involved in the regulation of autophagy also regulate apoptosis. The molecular reg-ulators of both pathways are interconnected;...

Báo cáo khoa học: Identiﬁcation and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt

... 2957Identiﬁcation and characterization of a collagen-inducedplatelet aggregation inhibitor, triplatin, from salivaryglands of the assassin bug, Triatoma infestansAkihiro Morita1, Haruhiko Isawa2, ... or absence of 1.0 lM triplatin-1 and analyzed by anti-FcR c-chain(aFcR c-chain) and antiphosphotyrosine (aPY) immunoblotting. A. Morita et al. Identiﬁcation and characterization of triplatinFEBS ... a 2b1 and a IIbb3integrins and secretion of platelet agonists suchas ADP and thromboxane A 2, accelerating plateletaggregation and thrombus formation. Therefore, GPVIhas a central role in the initial phase...

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