Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies ”" pptx

Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx

... Access “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies. ”Hajib ... al.: “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies. ”. ... among asymptomatic and symptomaticpatients respectively [11]. The utility of the real time PCR technique f or HCMV infection and usefulness of both the technique in detect ion of symptomatic...

Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx

... wild-type and vaccine strains. In summary, the multiplex RT-nPCRdeveloped in this study is a highly specific and sensitiveassay for the rapid detection and differentiation of wild-type and vaccine ... difficult and necessary to discriminatebetween wild-type and vaccine strains because the atten-uated CDV vaccine is used widely in China. So a method to specifically detect the wild-type CDV strains ... wereassayed by RT-nPCR to determine its sensitivity. RT-nPCR was performed to identify cells infected with CDVvaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, field...

Báo cáo y học: "A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients" pdf

... cohorts and the sensitiv-ity and specificity of the detection methods [10-16]. The majority of mutations in imatinib-resistant patientsusually occurred within the nine amino acid positions of KD including ... 9 and 22, creatinga new fusion gene, BCR-ABL, with constitutive t yrosinekinase activity [2]. Targeting BCR-ABL- transfe cted celllines and murine CML models with a variety of tyrosinekinase ... early as possible to detect the lowest levels of the mutant clone [24,25]. In this study, we set out to develop a single-tube allelespecific -polymerase chain reaction (AS-PCR) to identifythe...

Báo cáo y học: "Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus" potx

... reversetranscriptase (Invitrogen) and 40 U RNase inhibitor. Atotal of 20 μL of the mixture was initially incubated at25°C, then the reaction was held at 65°C for 60 min and finally it was terminated by incubation ... were found to be closely related to strains belong-ing to the Asia-1 lineage, which is distant and phylogenet-ically distinct from the vaccine strains. Additionally, the analysis of the three ... swabs with the clinicalsuspicion of canine distemper provided by the VeterinaryTeaching Hospital of National Chung Hsing University and by the Taichung City Animal Protection and HealthInspection...

Báo cáo y học: "PTPN22 polymorphism and anti-cyclic citrullinated peptide antibodies in combination strongly predicts future onset of rheumatoid arthritis and has a specificity of 100% for the disease" ppsx

... from arginineinto tryptophan. This amino acid change disrupts the binding of Lyp to an intracellular kinase, Csk, which can then no longerinactivate another kinase, Lck, that is involved in T-cell ... a main investigator and carried out the genotyping, the statistics and contributed to preparation of the manuscript. LÄparticipated in the collection of the material and registration of the ... in an animal model [17] and in human cell lines[5]. The functional effect of the PTPN22 1858 polymorphismon T-cells in humans is yet to be demonstrated. The Lyp pro-tein is expressed in other...

Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx

... real- time RT-PCR assay.Intrinsic performance of the duplex real- time RT-PCR assayLinearityLinearity of the duplex real- time RT-PCR assay wasdetermined using serial 10-fold dilutions of a clinicalsample ... B)assays in the testing of the same sample with of low load of HCV.Assay of 109 serum samples by using commercial kits The results obtained by the duplex real- time RT-PCRassay were identical to ... harvested and quantified. In order to determine the optimal concentration of ICused in the duplex real- time RT-PCR assay, the armoredRNA was serially diluted and then spiked into the national...

Báo cáo y học: " Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD" pot

... similar to the study of Laitinen et.al. S ince we ana-lyzed the immunohistochemical expression of Tn-C in the specific histological localizations of the airwaymucosa instead of measuring it. F urthermore, ... was to quantify measures of remodeling in the large airways in COPD compared to smokers and nonsmokers. We therefore hypothesizedthat the expression of Tn-C is inc rease d in COPD simi-larly to ... patients with COPD. The clinical relevance of our finding can only bespeculated. Hypothetically, increased ECM deposition in the large airways in our COPD patients may contri-bute to airways obstruction....

Báo cáo y học: " B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both " pot

... against TM region of HERV Envs have beensuccessfully used in immune assays including flow cytom-etry and immunofluorescence staining by others, as theyare able to detect the TM region of a ... expres-sion, thereby probably diminishing the immune reactivity and thereby also influence the pathogenesis of the actualMS attack. The role of HERV-expressing monocytes is more uncertain and not ... crucial in the development of some types of demyelinating lesions [48-53]. The autore-active CD4+ T cells in MS may be activated in the periph-ery and once activated, they can cross the blood-brainbarrier...

Báo cáo y học: " G140S/Q148R and N155H mutations render HIV-2 Integrase resistant to Raltegravir whereas Y143C does not" potx

... resistance to RAL emerges rapidly both in vitro and in vivo,through the selection of mutations within the IN coding region of the pol gene, greatly reducing the susceptibility of the virus to the inhibitor. ... suggestingpossibledifferencesintheresponseoftheenzymestoinhibitors and in the mechanisms by which resistanceemerges. In this study, we characterized the in vitroactivity of HIV-2 IN produced from the IN codingregion ... activity in v itro, for both the 3’-processing and strand transfer activities, by comparing the efficiency of IN activities with that of the wild-type reference N1enzyme. HIV-2 IN harboring the...

Báo cáo y học: "Impaired glucose and nutrient absorption in critical illness: is gastric emptying only a piece of the puzzle" docx

... most of them fed postpylorically [7]; in 13 patients with a faecescollector because of loose stools, the caloric value of energyloss was a mean of 301 kcal/day, and 3 patients had a loss of more ... kcal/day in the stools [7].CommentaryImpaired glucose and nutrient absorption in critical illness: isgastric emptying only a piece of the puzzle?Alain DiveDepartment of Intensive Care, Louvain ... during critical illness [2]. They analysed the kinetics of glucose absorption, glycaemia modifications, and gastric emptying after a test meal in 19 critically ill patients, with comparison to...

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