Báo cáo y học: "Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain (D190A) that occurred upon virus transmission from turkeys to pigs" potx
Báo cáo y học: "Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain (D190A) that occurred upon virus transmission from turkeys to pigs" potx
... wasemployed to evaluate the cross reactivity between paren-tal(190Asp)andHA-mutant(190Ala)TK04viruses.Additionally, cross reactivity was evaluated betweenTK04 parental and mutant viruses, and other ... turkey H3N2 influenza isolates. More work is needed to evaluate the replicationand antigenicity of 190Ala mutation in vivo . Addition-ally, it is of importance to see the e ffect of the above mutation ... DA, Suarez DL: Generation of reassortant influenza vaccines by reverse genetics that allows utilization of a DIVA(Differentiating Infected from Vaccinated Animals) strategy for the control of...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... GTTGTCGACAAACATCTCAACTAG-3 and GSTX3: 5¢-GTAAGTGTGGGAATAAGATCAAATC from adult S. mansoni reverse-transcribed RNA. The product of the PCR was sequencedand used as probe to screen an adult worm ... blot analysis was performed in order to ascertain that the cloned sequence belonged to the parasiteand was not due to an artifact, and also to examine the copynumber of SmGSTO genes in the parasite ... Branchlengths are proportional to estimates of evolutionary change. The number associated with each internal branch is the local bootstrap probability that is an indicator of confidence. The sequences are...
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Báo cáo y học: "Characterization of an H10N8 influenza virus isolated from Dongting lake wetland" potx
... The viralpolymerase mediates adaptation of an avian influenza virus to a mammalian host. Proc Natl Acad Sci USA 2005, 102:18590-18595.19. Hatta M, Gao P, Halfmann P, Kawaoka Y: Molecular basis ... important component in the transmission cycle of avian influenza virus. Monitoring the water at aggregation and breeding sites of migratory waterfowl, mainlywetland, is very important for early ... Monitoring the water at aggregation andbreeding sites of migratory waterfowl, mainly wetland, isvery important for early detection of avian influenza virus [3]. Dongting Lake wetland is an important...
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Báo cáo y học: "Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element" ppt
... 5′-agcttttccaaaaaaagctcatcggaactgacaatctcttgaattgtcagttcc-gatgagctg-3′; PRE 1329 5′-gatccgctgacaattctgtcgtcctttcaa-gagaaggacgacagaattgtcagttttttggaaa-3′ ; AtPRE1329 5′-agcttttccaaaaaactgacaattctgtcgtccttctcttgaaaggacgaca-gaattgtcagcg-3′. ... amplificationoftheSAsequence(forward:SAF_XhoI5′-gaattcctcga-gagaccaatagaaactgggc-3′, reverse: SAR_EcoRV 5′-gaattc-gatatccctgtggagagaaaggcaaagtg-3′).Amplification of the deletion series of the PREThree ... blot analysis with primary antibodies: anti-GAPDH, anti-firefly luciferase protein (anti-Luc) and anti-Poly A binding protein (anti-PABP) and the horseradish peroxidase-conjugated secondary antibody.Panjaworayan...
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Báo cáo y học: "Characterization of vascular strain during in-vitro angioplasty with high-resolution ultrasound speckle tracking" ppt
... strain was calculated as the gradient of the longitudinal displacement(derivative of the displacement) along the ultrasound beam, and the shear strain was cal-culated as the partial derivative ... 11Since angioplasty is a common treatment for s tenosis and results in ch anges in th earterial fiber anatomy of the tunica media as the angioplasty balloon expands, weinvestigated the ultrasound ... in a region of the artery to estimate the radial normal strain. All strain values were measured in the axial direction, where resolution is at least an order of magnitude greater than that in the...
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Báo cáo y học: "Attenuation of murine antigen-induced arthritis by treatment with a decoy oligodeoxynucleotide inhibiting signal transducer and activator of transcription-1 (STAT-1)" ppt
... cellular activation, proliferation and differ-entiation by means of the janus kinase/signal transducer andactivator of transcription (JAK/STAT) pathway [5,6]. The bind-ing of these cytokines to ... of the acute phase of AIA. These dataimply that activation of the STAT-1 pathway plays an importantrole in our Th1 cell-mediated arthritis model.Th1 cell function is already an essential pathogenic ... expression analysis and provided the STAT-1 decoy ODN. KT did the statistical analysis. MHe andRB designed and coordinated the project. All authors readand approved the final manuscript.Available...
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Báo cáo y học: "Characteristics of HIV-infected women and factors associated with HCV seropositivity in the Republic of Georgia" doc
... viral hepatitis, commercial sex work, riskfactors of the patient’s last regular sexual partner(s), andmedical care-related risk factors (e.g., history of invasivemedical manipulations and ... identified from the HIV/AIDS surveillance database. HCV status was availablefor 244 women. Because of substantial missing data,analyses were genera lly limited to 228 women. Women with complete and ... relationship: the majority were HIV positive (84%) , nea rly 69% gave a h is-tory of IDU, and approximately 66% had documentedHCV antibodies. About 30% of women had a history of an STI. Approximately half...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... of pravas-tatin is open hydroxyl-acid so that its hydrophilicity is markedly higher than that of other statins. The oral bioavailability of this statin is low due to degradation in the stomach ... Magnification is X5000. Figure 4, Percent of pravastatin and hemoglobin release from loaded erythrocytes in PBS and plasma. Data were tested by one-way analysis of variance and represented as mean ... they are used in the treat-ment of many diseases such as osteoporosis, Alz-heimer disease, organ transplantation, stroke and di-abetes [1]. Administration of statins by oral rout is associated...
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Báo cáo Y học: Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid doc
... coevaporation with methanol, the mixture of partially methylated alditols was acetylated with acetic anhydride (3 h, 120 °C), and analyzed by GLCand GLC–EIMS [14,19].NMR spectroscopyPrior to NMR analysis, ... spectrometric and NMRanalyses of the oligosaccharide obtained from the polysac-charide by de-N-acetylation followed by deamination andreduction demonstrated the ÔheptaÕsaccharide repeating unit to be:in ... eV.Monosaccharide and methylation analysisFor monosaccharide analysis samples were subjected to methanolysis (methanolic 1MHCl, 18 h, 85 °C), followedby re-N-acetylation and trimethylsilylation...
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Báo cáo y học: "Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation"
... Edamoto 5, Naoto Aiba 6 and Tetsutaro Sata 1 1. Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan 2. Department of Transplantation Surgery, Nagoya University ... Hospital, Aichi, Japan 3. Department of Transplantation Surgery, Kyoto University Hospital, Kyoto, Japan 4. Institute of Biomedical Research and Innovation, Hyogo, Japan 5. Department of Surgery, ... International Medical Center of Japan, Tokyo, Japan 6. Aiba Clinic, Saitama, Japan Correspondence to: Dr. Kenji Abe, Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1,...
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