... E22Q and D23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and fibrillation. ... buffer supplied with the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The ... cut-offcentrifugal filtration device. When a solution of Ab(M1–40) of approximately 40 lm was concentratedapproximately eightfold, approximately 75% of thepeptide was recovered at a concentration...