Báo cáo y học: "Molecular characterization of partial-open reading frames 1a and 2 of the human astroviruses in South Korea" doc

... Virology Journal 20 10, 7 :22 1http://www.virologyj.com/content/7/1 /22 1Page 3 of 5SHOR T REPOR T Open AccessMolecular characterization of partial-open reading frames 1a and 2 of the human astroviruses ... characterization of partial-open reading frames 1a and 2 of the human astroviruses in South Korea.Virology Journal 20 10 7 :22 1.Submit your next manuscript to BioMed Central and take full advantage of: • ... forRT-PCR. The Mon340 and Mon348 primers were usedfor the amplification of the ORF1a region, and the Mon269 and Mon270 primers for the amplification of ORF2 (Table 1). For the synthesis of cDNA,...

Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf

... Expression of galactokinase driven by the CYC1 promoter in the wild-type strain and the M RG19 disruptant. Transformants (in tripli-cates) of wild-type and MRG19 disruptant bearing either vector(control) ... metabolize the carbonsource (Fig. 6, Compare 1 and 2 or 3 and 4) However, anincrease of 50% in the rate of oxygen uptake wasobserved in the MRG19 disruptant as compared with the wild-type in the ... in MIG1 disruption(Fig. 10, lane 1 and 2) and wild-type (Fig. 10, lane 3 and 4) strains bearing multiple copies of MRG19 and grown in the presence of glucose (Fig. 10, lane 1 and 3) and glycerol(Fig....

Báo cáo y học: "Molecular characterization of partial-open reading frames 1a and 2 of the human astroviruses in South Korea" ppsx

... Whitefly-transmitted geminiviruses and associateddisorders in the Americas and the Caribbean basin. Plant Dis 19 92, 76 :22 0 -22 5.11. Morales FJ, Anderson PK: The emergence and dissemination of white-flytransmitted ... Mol Biol Evol 20 07, 25 :23 9 -24 2.30. Posada D, Crandall KA: The effect of recombination on the accuracy of phylogeny estimation. J Mol Evol 20 02, 54:396-4 02. 31. Falush D, Wirth T, Linz B, Pritchard ... 88:3458-3468. 27 . Prasanna HC, Rai M: Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia. Virol J 20 07, 4:111. 28 . Awadalla P: The evolutionary genomics...

Báo cáo y học: "Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece" pptx

... of furthe r virus spreadingwithin the community [11]. Analy sis of the environmen-tal and clinical isolates of our study showed the pre-sence of HAV strains belonging to genotype IA. SubtypeIA ... pseudoreplicates), using MEGA 4.0 .2 software. The HAV genotype was determined by comparing the different sequences of the Gree k strains included in the phylogenetic analysis with the reference sequences of different ... epidemiology of viral infections circulating in the com-munity, and at the same time reveal the occurrence of asymptomatic infections in the population [6].Conclusions In conclusion, this study - the...

Báo cáo y học: " Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1" docx

... participated in coordination of the study. All authors read and approved the finalmanuscript.Competing interests The authors declare that they have no competing interests.Received: 12 July 20 10 Accepted: ... 4software following published protocols [21 ,22 ]. The amino acid sequence of endosialidase was determined and compared with the protein structure family databasesPDB [23 ], SCOP [24 ,25 ], and ... Protein Data Bank.Nat Struct Biol 20 03, 10:980. 24 . Andreeva DH, Brenner : SCOP database in 20 04: refinements integratestructure and sequence family data. Nucleic Acids Res 20 04, 32: D 226 -D 229 . 25 ....

Báo cáo y học: " Molecular characterization of porcine circovirus 2 isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus" pot

... 13S 92 23V 12I134 9N 36T151 28 T 17P169 10R 33S 2G190 19T 14S 12A191 5R 13A 27 G 20 6 30I 15K 21 0 13E 14D 21 5 8I 37VORF3 29 34T 11A 40 12 R 10S 23 G88 41Q 4E100 25 F 20 L101 9Y 9N 6D 21 H1 02 ... sequence of strain AY691679, using the Primer 5.0 software. The sequences of the primers* Correspondence: yijianzhong@yahoo.comInstitute of Animal Husbandry Veterinary Sciences, Shanghai Academy of Agricultural ... pro-tein, which is the main structural protein and also the major antigen inducing neutralizing immune responses[6]. The ORF3 p rotein is involved in PCV2- inducedapoptosis by the caspase-8 and...

Báo cáo y học: " Molecular characterization of two distinct monopartite begomoviruses infecting tomato in india" pps

... region of New Delhi, India in the middle of February 20 06 and from the fields of Kerala (a southern state of India) in April 20 08. Due to the association of whiteflies with the plants, begomovirus infection ... map of the virus (Figure 2C). The degree of relation-ship of the nucleotide sequence of Intergenic Region (IR) and the amino acid sequences of the proteins expressedby the viral genomes of the ... collected from the In dian subcontinent in an effort towards identifying and determining the molecular variability of ToLCVs. In view of evolution of variants of Begomovirus family of plant viruses,...

Báo cáo y học: " Molecular characterization of a Chinese variant of the Flury-LEP strain" potx

... strains.Protein FluryLEP Flury-LEP-C Hep-FluryN Y2 88 H288 H288PD59G59D59F115 L115 L115L186 W186 W186K231 E231 E231M A 22 V 22 A 22 GV13G13V13H283 R283 R283Q297 K297 K297E368 G368 E368 Y3 71 H371 ... vaccine strain maintained in BHK -21 cells; derived from the vaccine strain FluryLEP FJ577895FluryLEP Vaccine strain DQ099 524 Hep-Flury Vaccine strain derived from the vaccine strain FluryLEP ... foruse in China as a vaccine strain [10 ,22 ], and the result in our study also supported the hypothesis.Table 3: Different proteins of Flury-LEP-C compared with FluryLEP and Hep-Flury strains.Protein...

Báo cáo y học: " Molecular characterization of genome segments 1 and 3 encoding two capsid proteins of Antheraea mylitta cytoplasmic polyhedrosis virus" pps

... protein of 121 0 amino acids with molecular mass of ~137 kDa.BLAST analysis showed 20 -22 % homology of S1 and S3 sequence with spike and capsid proteins, respectively, of other closely related cypoviruses ... Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dyebinding. Analytical Biochemistry 1976, 72: 248 -25 4.41. Harlow ... transcription, possessing all the enzymesnecessary for mRNA synthesis and processing [8].BmCPV, the type Cypovirus, has a single layer capsidmade up of 120 copies of the major capsid protein,* Correspondence:...

Báo cáo y học: " Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia" pot

... sub-genotype VIIb strains isolated in Southern Africa mighthave originated in the Far East as their lack of diversityindicated that they cannot be indigenous to South Africa[8]. The finding in this ... 0.0 32 0 .24 2 0.1 32 MB093/05 34 9 0.0 32 0 .24 2 0.1 32 MB095/05 35 9 0.0 32 0 .25 2 0. 127 MB 128 /04 34 9 0.0 32 0 .24 2 0.1 32 MB085/05 18 4 0.014 0.134 0.104* compared to the consensus nucleotide and amino ... Africa and are endemic in SouthernAfrica [8,11,13]. The presence of genotype VIII virusneighbouring country Singapore in early 1960 s and in Malaysia (AF 224 0) that gave rise to the recent genotypeVIII...

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