... surface at 4 °C was efficiently endocytosed after15 min at 37 °C. After 30 min, the pool of intracellularantibody had decreased to 25%, and, at 60 and 90 minafter internalization, the antibody ... expression of Sial-T2–hemagglut-inin (GD3 synthetase) in the same membrane was analyzed as a control of protein loading. IgG (L), Light chain of IgG. Scale bars: 20 lm.Endocytic trafficking of an antibody ... Mountain View, CA,USA) [6].Cell labeling and internalization assaysCells transiently transfected or not with plasmids indicatedabove were incubated on ice for 10 min to inhibit intra-cellular...