... histologically and two were proc-essed for mRNA analysis of the SFB layer and cartilage. For each experimental parameter, patient SFB were analysed sep-arately for each donor. After 14 days of in ... with primaryantibodies against prolyl-4-hydroxylase (Biomeda, Foster City,CA, USA), Thy-1 (CD90; Dianova, Hamburg, Germany),COMP (rabbit polyclonal antibody directed against humanand bovine ... describing the induction of MMP-1 and MMP-3 in cartilage and SFB by TNF-α and/or IL-1β [42,43]. Therefore, they support the validity of the new model for the analysis of the mechanisms of cartilagedestruction...