báo cáo khoa học: " Substance abuse and pharmacy practice: what the community pharmacist needs to know about drug abuse and dependence" ppsx

... practice: what the community pharmacist needs to know about drug abuse and dependenceAnthony C Tommasello*Address: University of Maryland School of Pharmacy, Office of Substance Abuse Studies, ... category are sufficient to addressmany inquiries. Furthermore, drug users themselves can-not be certain that the drug they bought is the drug theyset out to purchase. The illicit drug market provides ... pharmacists to assist in the clinical management of opioid and other drug dependencies. Pharmacists need to be well informed about issues related to addiction and preparednot only to screen, assess, and...

Báo cáo khoa học: Aegyptin displays high-afﬁnity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

... ka1 and ka2rates. On the otherhand, the off-rates, kd1, for the (GPO)10 and GFO-GER interactions with the inhibitor were approxi-mately 100-fold faster relative to collagen and the RGQOGVMGF ... Bio-Sciences,Uppsala, Sweden) and cloned into a VR2001-TOPO vector(modiﬁed version of the VR1020 vector, Vical Inc., SanDiego, CA, USA), and their sequence and orientation wereveriﬁed by ... several receptors, and the relative contribu-tions of each of them have been intensely investigated[3–5]. The initial tethering of platelet to the extracellu-lar matrix is mediated by the interaction...

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... without the interference of auto-proteolysis, the addition of inhibitors to the enzymeswas examined. Inhibitors such as phosphoramidon oro-phenanthroline are fluorescent and disturb the applied ... unfolded and the degraded protein are nearly identical [15], the progresscurves in the presence of GndHCl reflect the decrease ofnative molecules, and autoproteolysis does not disturb the measurements.Kinetic ... unfolding and autoproteoly-sis were started by the addition of protein to the GdnHCl-containing solution. The progress curves were fitted to asingle exponential function, yielding the rate constantskobs.AcknowledgementsThe...

Báo cáo khoa học: Increased susceptibility of b-glucosidase from the hyperthermophile Pyrococcus furiosus to thermal inactivation at higher pressures pptx

... addi-tion, the band at  1.0 GPa in Fig. 5C does notresemble the broad, featureless band typical of anunfolded protein. Taken together, these observationssuggest that the unfolding at the level of the ... on the ther-mal stability, revealing that the secondary structure of the enzyme remains intact up to the upper limit of the investigated temperature range (99 °C) [1,4] To ourknowledge the ... suchas salt bridges and ion pairs than in the case of the enzymes from the hyperthermophiles S. solfataricus and Thermosphaera aggregans [4]. Electrostatic interac-tions are known to be very pressure...

Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx

... on their capacity to induce the production of proinflammatory cytokines, to pro-mote the recruitment of dendritic cells and monocytes to the site of injury, and to enhance phagocytosis and the ... considered to bemultifunctional molecules. They stimulate the produc-tion and release of proinflammatory and anti-inflam-matory molecules, they recruit inflammatory cells to the site of injury, they ... combina-tion of these peptides and cytokines present at the siteof injury will contribute to the maturation of theseimmature dendritic cells, enabling them to processantigen and to migrate to proximal...

Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... in the DRE motif by all four of the different AtERFs.On the other hand, some other residues reported to determine the geometry of the a-helix relative to the b-sheet were not as conserved as these ... 1B), from the promoter region of the tobacco Gln2 gene [3] and the Arabidopsis rd29A gene [19],respectively, were prepared, together with their mutants, bysynthesizing both stands. The EMSA, ... by the CaMV 35S promoter, together with the luciferasereporter gene containing four tandem copies of either the DRE motif or its mutants at the upstream regula-tory region, was coexpressed into...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... however, to achieve these results, it wasnecessary to subdivide several of the mutation datasets. For example, for the MS2 complex, the mutationdata had to be divided into two classes based on the presence ... absence of a cytosine at position )5 in the RNA. A likely explanation for the importance of the )5 cytosine mutation is offered by the observationthat the amino group of the cytosine at position ... structures, together with the availability of dataon speciﬁcity, will further improve the performance of the knowledge-based predictive method presented here.We retained the all atom representation...

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... and FUGUE [60] and gave the same results, therefore confirm-ing the correctness of the chosen templates. The sequencesof target and templates were aligned to each others and to those of the first 30 ... and adenine, inosine and hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and 4.2 min and 6.6 and ... 2008 The Authors Journal compilation ª 2008 FEBS To explore the correlation between the resistance to proteolysis and the conformational protein stability and to obtain information about the flexible...

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... a-catenin and b-catenin assemble the cytoplasmic cell adhesioncomplex (CCC) on its intracellular domain and linkE-cadherin indirectly to the actin cytoskeleton [3].Through the establishment of the ... scattering and the redistribution ofE-cadherin into the cytoplasm. However, the mechan-ism of Src activation by Decma treatment has not beenelucidated. The actin cytoskeleton seems not to benecessary ... growth factor receptor-binding protein 2 (Grb2) and results in the recruitment of the Grb2–son ofsevenless (Sos) complex to the vicinity of Ras, whereSos acts as a GTP exchange factor for Ras....

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... individuallymutated to L eu (sbwAFP ) or to Tyr (TmAFP) and t he TH activi ty o f the p rotein was measure d. The top of the panel sho ws the protein with the T XT face oriented to wards the viewer, while the ... r otation about the v1dihedral angle was observed, and analysis of the Cbatoms of t he TXT threonines f ound them to be asmotionally rigid as the backbone [60]. Taken together, theseexperiments ... Graether and B. D. Sykes (Eur. J. Biochem. 271) Ó FEBS 2004[22–24]. These results weak en the hypothesis t hat the Thrface of the a-helix is critical to the ice-binding interaction.Furthermore,...

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