... 8 kDa [6,29,40,42].Therefore, the amount of both fragments is indicativeof the amount of the red form. Cleavage occurs on asubmillisecond time scale, as shown for single EosFPmolecules ... Immunoblot analysis of the red-emitting form of mcavRFP. Averageoptical density and standard deviations were calculated for the 20 kDa band of the red form, taking measurements from four independent,zooxanthellae-free ... animals, which were exposed tolight for the same time interval, displayed constantoverall red fluorescence of the tissue.Prolonged darkness is stressful for the corals becausethe light-deprived...