... [Ca2+-freebasal salt saline (BSS)]. In the presence of 2 mm extra-cellular calcium, mAChR activation induced both a transient and a sustained increase in intracellularcalcium, as measured by Fura 2 ... phosphatidylinositolresponses in brain and salivary glands. Biochem J 206,587–595.9 van der Wal J, Habets R, Varnai P, Balla T & Jalink K(2001) Monitoring agonist-induced phospholipase Cactivation ... Gra¨felfing,Germany) and a C6790 CCD camera (Hamamatsu Pho-tonics, Hamamatsu, Japan), while the images in Figs 5 and 6 were obtained using an IX70 inverted microscope,fitted with an OSP-EXA...