... Liu LM, Wang YF, Wang LY & Qiao ZD (2006)Characterization and quantification of mRNA tran-scripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression. Human Reprod ... Developmental Genetics and NeuropsychiatricDiseases (Ministry of Education), Shanghai Jiao Tong University, China2 Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, ChinaIntroductionThe ... at 4 °C, and remove the supernatants. Dry the pellets in air and dissolve in 10 lLH2O.Step 10. Sequencing GLGI clonesPerform the sequencing reaction in a total volume of 7 lL (including 4 lL...