báo cáo khoa học: "Local suffering and the global discourse of mental health and human rights: An ethnographic study of responses to mental illness in rural Ghana" pptx

... chosen to stay and serve the pastors who had formerly chained them, training aspastors themselves and doing other work such as farming,maintenance and running errands. In turn they tooassisted in ... the history of the person's illness, the symptoms and course of the illness, possiblecauses, the impact of the illness on the individual and the family in terms of day -to- day life and social ... to address the factors which contribute to the con-tinued use of chaining and other forms of abuse, and work alongside families and healers to protect and pro-mote the rights, dignity and health...

Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence speciﬁc DNA binding proteins pptx

... migrating 70- and 68-kDa cross-linkedproducts may represent the same protein bound to the H and L-strands of the probe. Some of the discrepanciesbetween the South-western and UV cross-link analyses ... partial termination past the rRNA genes, details as to the specific site and the mechanism of termination of the H-strand distal gene transcription remain to be elucidated.Insight into the possible ... sequencespecificity of protein cross-linking.Purification of D-TERM binding proteinsWith a view to understand the nature of D-TERM DNAbinding proteins and their possible role in transcriptionFig....

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... However, the ﬂuores-cence in the mutants without tryptophan at 140(W140A and W140O) was much lower than both the wild-type protein and mutants E142O and K133A.Thermal analysis of protein unfolding The ... located in the C-terminal loop of SNase plays an important role in protein stability and conforma-tional integrity. During protein unfolding ⁄ refolding, the addition of 2.5 protons (both E75 and ... exam-ined by subjecting these mutants and wild-type pro-teins to CD [11,12] and differential scanningcalorimetry (DSC) [13,14]. The preliminary refoldingprocess in terms of assembly of fragments...

Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20mutants are given in Table 1. The DHcal and Tm of the wild-type protein ... exists in these mutants (Fig. 1).Thermal analysis of protein unfoldingThermal denaturation determined by DSC was used to measure the stability of the mutant proteins. Based on the measurements of ... shown). Therefore, we hypothesize that pHunfolding SNase is mainly processed by the addition of 2.5 protons and both E75 and E129 are the targets of protonation.It is of interest to determine which...

Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx

... of analogs in the active center. Furthermore,these ﬁndings also seem to indicate that the analogs canFig. 5. Scheme of the HDC reactions with the natural substrate histidine and the histidine-analogs ... Pauling in 1948 and the laterformulation of the induced-ﬁt hypothesis by Koshland, it iswell established that the interactions between an enzyme and its substrate induce conformational changes ... inhibitor was not able to protect the enzyme against thermal denaturation. The increased resistance of the a-FMH-treated protein to thermal denaturation would indicate that the covalentbinding...

Báo cáo khoa học: " Local tumor control and toxicity in HIV-associated anal carcinoma treated with radiotherapy in the era of antiretroviral therapy" pps

... fractionation of mitomycin C in locallyadvanced anal carcinomas. Results of a phase II study of the European Organization for Research and Treatment of Can-cer. Radiotherapy and Gastrointestinal Cooperative ... Role of mitomycin in combination with fluorouracil and radiotherapy, and of sal-vage chemoradiation in the definitive nonsurgical treatment of epidermoid carcinoma of the anal canal: results of ... treatment of locally advanced anal cancer: results of aphase III randomized trial of the European Organization forResearch and Treatment of Cancer Radiotherapy and Gas-trointestinal Cooperative...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... process), and the further differentia-tion of the whole organism into two distinct phenotypes,are common throughout the animal, plant and fungikingdoms. Nevertheless, with respect to animals ... how the initial molecu-lar activity of Sry is translated into cellular structure and organ morphology. Interestingly, Dmrt1, the ancestor of Dmrt1bY, is one of the downstream effec-tors of Sry ... shown to be the major male and female determinants, respectively. Altogether, itappears that when earlier in the cascade of sex deter-mination, the role of Dmrt1 is ﬁrst to be an inducer of sex...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... Facility of Tianjin MedicalUniversity Cancer Institute and Hospital and the National Foundation of Cancer Research for provid-ing human gastric tissue samples. We also thank the College of Public Health ... vector bearing the potential miR-23a binding site in the ﬂuorescentreporter, and found an increase in EGFP intensity afterblocking miR-23a and a decrease in EGFP intensityafter overexpressing ... secondaryantibody. Protein expression was assessed by enhancedchemiluminescence and exposure to chemiluminescent ﬁlm.LabWorksÔ Image Acquisition and Analysis Software wasused to quantify the band intensities....

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the activation may be a result of the interactionbetween the inhibitory region and ... 116–131). In the relaxed state, the inhibitory region binds to actin and inhibits actin–myosin interaction [11,12],while in the contractile state, Ca2+-binding to site I and ⁄ or II of TnC causes the ... redissolved in equivalent volumes of 5M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+;...

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform speciﬁcity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... the study of the C. elegans MT system at the protein function level to be of the highest interest, in order to shed light on the possible physiological functions of MTs in thisorganism and to ... as the contri-bution of CeMT2 to ingested cadmium detoxiﬁcation.Results and DiscussionIdentity and integrity of the recombinant CeMT1 and CeMT2 polypeptidesRecombinant synthesis from the ... issensible to deduce that the coordination of the Zn(II)ion bound at the C-terminal moiety of CeMT1 is con-tributed to by histidines, and the number of theseinvolved in metal binding is analyzed...

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