báo cáo khoa học: ""I washed and fed my mother before going to school": Understanding the psychosocial well-being of children providing chronic care for adults affected by HIV/AIDS in Western Kenya" pdf

... to be critical in coping and promoting psychosocial well-being [42,43]. In line with Antonovsky's salutogenic approach and to broaden our understandings of the psychosocial well-being of ... affected by HIV/AIDS. In the absence of working adults in their households, many of the children were responsible for the generation of food and income to sustain their household. Alongside theseresponsibilities, ... change when her mother was admitted to the hospital. In the absence of adequate nursing care at the hospital, Carolyne continued to care for her mother in the hospital, forcing her to leave school....

Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through speciﬁc protein interactions in the methionine synthase–methionine synthase reductase complex docx

... footnote) in the reactionmixture may reduce a proportion of the FMN domain,converting some of the enzyme to the active form. The  3-fold increase in activity may arise from the binding of the FMN ... addition of 1 mmhomocysteine, and then further incubated at 37 ° C for 10 min. The reaction was quenched at 90 °C for 3 min, and the reaction mixture was then cooled to room temperature before being ... prior to introduction into the glove box. A concentrated sample of MSR or the isolated FMN domain was introduced into the glove box, and FeCN was added to the concentrated pro-tein stock to fully...

Báo cáo khoa học: Metabolomics, modelling and machine learning in systems biology – towards an understanding of the languages of cells potx

... including creating it, storing it, editing it, com-paring it with other stored models, ﬁnding it again in a principled way, visualizing it, sharing it, running it,analysing the results of the ... many and they act in nonlinear ways). By contrast, making mathematical models of the biologi-cal systems one is investigating (and seeing how theyperform in silico) is generally considered a minoritysport, ... these have yet to be identi-ﬁed chemically. The history of biomedicine as perceived via the awards of the Nobel Committee indicates the import-ance to our understanding of the subject of both smallmolecules...

Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt

... 90 s, followed by acetone for 1 min, before rinsing in distilled water. The slides were then stained for 5 min in a toluidin blue solution(0.1% toluidin blue, 1% borax; pH 11), rinsed with distilledwater ... nt250436–253041) to obtain pJL265. This fragment includes exon 0 of phk-2, the ﬁrst intron, and the ﬁrst exon to the ATG, which is used to initiate translation of GFP S65T. The transformingvector pJL264 ... Jousset and Max Bergoin for providing virus stocks and much useful advice in the early stages of thisproject and Liliane Gloeckler and Anne-Marie Aubertin for assistance in producing virus stocks;...

báo cáo khoa học: " LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus?" ppsx

... Bovine Serum, 100 units/ml of penicillin and 100 μg/ml of streptomycin. Cytospins of the OE-33 cell line were fixed in acetone and dried for 10 minutes. Rehydration, blocking, and the staining ... in intestinal metaplasia and EACs may thus sustain multi-step carcinogenesis. Ourfindings seem to be very well in line with current under-standing of carcinogenesis according to an integratedmodel ... in the design of the study, evaluated cancer samples, and helped to draft the manuscript. LM participated in the design of the study and performed RT-PCR studies. CR and LS participated in the...

báo cáo khoa học: " Fever, hyperglycaemia and swallowing dysfunction management in acute stroke: A cluster randomised controlled trial of knowledge transfer" pot

... patientperformance in 10 activities of daily life. The items aredivided into groups that relate to self -care (feeding,grooming, bathing, dressing, bowel and bladder care, and toilet use) and a ... those ASUs randomised to the interventiongroup. Following a minimum period of three months to allow the FeSS intervention to become an integral part of usual clinical practice in the intervention ... briefly outlining the study. Following this, CL and SM will meet face -to- face with the Medical Director and the NUM of each ASU to fully explain the study and obtain informed consent. To describe key...

Báo cáo khoa học: " A simple and rapid Hepatitis A Virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations" potx

... substitution in the 2B protein was used as the background to construct HAV-Bsd. The blasticidin resistance (Bsd) gene coding for the blasticidin deaminase was inserted into the 2A-2B junction of the infectious ... KK and MLVT par-ticipated in the design of the study. MYY and GGK con-ceived of the study, and participated in its design and coordination. KK and GGK drafted the manuscript with the help of ... on the antibioticresistance of HAV-infected cells conferred by the blastici-din deaminase gene inserted into the virus genome. In the presence of blasticidin, HAV-Bsd-infected cells continueSchematic...

Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... naturallyoccuring FtGLDHAA and the artiﬁcial AtGLDH1AA,are indeed exclusively due to the insertion of twoadditional alanyl residues into the N-terminus of the H-protein. In conclusion, the substrate ... method for producingrecombinant H-proteins leaves a small extension of four amino acid residues at the N-terminus of eachrecombinant protein after removal of the His tag (Fig.S4). The kinetic ... H-protein,FtGLDHAA, harbours two additional alanine residuesvery close to its N-terminus, and is by far the mostdominant H-protein type in leaf mitochondria of theseplants. Because of photorespiratory...

Báo cáo khoa học: New insights into Fragile X syndrome Relating genotype to phenotype at the molecular level docx

... analysisled to the suggestion that dFXRP is the ancestral pro-genitor of the vertebrate FMRP and FXR proteins, and that in ﬂies this single protein performs the func-tions carried out by the entire ... be to disrupt a ‘hydrophobic platform’involved in RNA recognition. They had observed sucha mode of KH domain–RNA interaction in the cocrys-tal structure of the KH3 domain of NOVA bound to an ... hypothesis of the deactivating -by- unfoldingeffect of I307N mutation using protein constructs con-taining the KH region of dFXRP.Results and Discussion The mammalian FMRP, FXRP1, FXRP2 and ﬂydFXRP...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... cloned into pDG148 vector to obtain the plasmid pOpac (Table 1). The respective sip(ba) geneswere isolated from initial cloning vectors and cloned into the vector pQE16 in frame with the His-tag-encodingsequence. ... 0.5, and then divided intocultures A and B, each of 5 mL. After i ncubation for 10 min a t either 3 0 °Cor42°C, the t wo cultures werelabelled for 1 min at the indicated temperature by the addition ... com-pared to the wild type and other sip(ba) mutant strain (datanot shown). The yields of protein s ecreted after 24 h of growth in TBY medium as well as the protein changedbanding pattern...

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