báo cáo khoa học: " Inversion variants in the human genome: role in disease and genome architecture" ppsx

Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc

... domainbinds to proteins containing the consensus sequenceD ⁄ E FPPPPXD ⁄ E [21–23]. The Pro-rich domain alsobinds to proﬁlin and proteins containing the SH3 and WW domains [17,24–26]. These ... that the EVH1, Pro-rich and EVH2 domains all have the potential to function in protein binding. The presentstudy revealed that the EVH2 domain is the RAD51-binding domain. Minimal RAD51 binding ... process, the ENA ⁄ VASP family proteins bind to both the barbed end and sides of actin ﬁlaments [15–18]. Theseactin-binding activities are conserved in the EVH2domain [19,20]. On the other hand, the...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... monomers. Human guanylate binding protein 5 (hGBP5) belongsto the family of interferon-c-induced p65 GTPases,which has seven members in the human genome [5].This family of guanylate binding proteins ... members of the human guanylate-binding protein family. J Interferon CytokineRes 27, 44–52.15 Rupper AC & Cardelli JA (2008) Induction ofguanylate binding protein 5 by gamma interferonincreases ... resulted in a2.5-fold increase in speciﬁc GTPase activity and increased formation of GMP during hydrolysis.Thermodynamics and stoichiometry of nucleotidebindingWe used ITC to investigate the thermodynamics...

báo cáo khoa học: " Inversion variants in the human genome: role in disease and genome architecture" ppsx

... Distribution of inversion variants in the human genome. The blue lines in this ideogram show the human chromosomal distribution of the 479 non-redundant inversion variants reported in the Database ... step towards a deeper understanding of human variation and genome dynamics.© 2010 BioMed Central Ltd Inversion variants in the human genome: role in disease and genome architectureLars Feuk*R ... of inversions in the human genome, the methods used to discover and type inversions, and their role in human disease and human genome architecture.Cytogenetically visible inversionsIt has long...

Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identiﬁcation of structural requirements for broad reactivity and speciﬁcity determinants for target proteases pptx

... the chagasin molecule is slanting towards the R domain. The angle of approach of cystatin B is )12.7°, and the inhibitor molecule is inclined towards the L domain of the enzyme. The difference in the ... are interpreted as furtherimproving the binding efﬁciency to papain and cathep-sin L versus the other enzymes, with the hydrogenbond between D99 in loop L6 of chagasin and S21e ofpapain providing ... equivalents in other enzymes), and the other (b) created by three Caatoms deﬁning the inhibitor framework and passingalong the inhibitory wedge. In the case of chagasin,plane (b) is triangulated by the...

Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx

... interactionsbetween the subunits and are involved in cofactorbinding, substrate binding and catalysis (Fig. 1). On the other hand, the mutations did not affect either the capability to bind the cofactor or the ... in orange, the oxygen atoms in red and the nitrogen atoms in blue. The helices forming the third cluster interact with the N-ter-minal helix of the other subunit in the dimer (shown in blue). ... contained in both equationswere shared (Fig. 2). The analysis gave a good globalﬁt of the data and showed that the midpoints of the sigmoid transitions coincide and, in the case of the L85A and...

Báo cáo khoa học: Acylation of lysophosphatidylcholine plays a key role in the response of monocytes to lipopolysaccharide ppt

... the LPCAT activity (data not shown). The stimulation ofLPCAT by IFN-c could be inhibited by the tyrosine kinaseinhibitor tyrphostin (2.5 lM) but not by the protein kinaseC inhibitor bisindolylmalamide ... LPS; inhibition of this enzymealmost completely inhibited LPS-induced TNF-a protein and mRNA production suggesting a role for LPCAT in the LPS-induced signaling pathways in monocytes. In addition,LPCAT ... added and the plate incubated for 20 min. The plate was washed and 100 lL of tetramethylbenzidine substrate containing0.01% H2O2(Kirkegaard and Perry, Gaithersburgh, MD,USA) per well. The...

Báo cáo khoa học: Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar doc

... LIP. The increase in ferritin by the iron preparations showed apattern of behavior similar to the increase of the LIP. The more iron appeared in the LIP the faster the synthesis offerritin took ... from the LIP is not stored in existing ferritin but is incorporatedinto newly synthesized ferritin. Compared to the increase in ferritin expression, the total amount of iron added to the cells in ... determined using the Bradfordmethod (Bio-Rad).Iron content of ferritinDuring the last step of the ferritin-ELISA (see above) the ferritin detaches from the surface of the wells and the ironcontent...

Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx

... 1.Histochemical staining of DBA (A and B), SBA (C and D), and Isolectin B4 (E and F) in the vomeronasal sensoryepithelium of the horse. The DBA (A and B), SBA (C and D), and Isolectin B4 (E and F) lectins ... Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ 17DiscussionThis is the first study to examine the binding of DBA,SBA, Isolectin B4, WGA, PNA, and UEA-I in the vomeron-asal ... weresituated in the lamina propria. Since the ducts of theseglands penetrated the epithelium and opened into the VNOlumen, the epithelium was covered with secretions from the Jacobson's glands,...

Báo cáo khoa học: "CT myelography of the thoraco-lumbar spine in 8 dogs with degenerative myelopathy" pptx

... class="bi x0 y1 w2 h 2" alt ="" ...

Báo cáo khoa học: " Multilobular tumour of the caudal cranium causing severe cerebral and cerebellar compression in a dog" doc

... cm in diameter concerning the right occipital, parietal and temporal bones and protruding from the right occipital bone (Figs. 1A and B). The mass severely compressed the cerebellum and the ... lobe (Figs. 1C and D) but did not appear to invade the brain tissue, the meninges or other neighbouring tissues. The mass was whitish to yellow and gritty on the cut surface. The lungs were ... compression 83tumour entities and conditions affecting bone [14], establishing, therefore, the correct diagnosis alters the prognosis and is paramount in pursuing the optimal therapeutic protocol....

Xem thêm