báo cáo khoa học: " Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp )" pptx

báo cáo khoa học: " Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.)" pptx

... Access Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp. )Christopher I Keeling1, Sabrina Weisshaar2, Steven G Ralph3, Sharon Jancsik1, ... 31:3381-3385.doi:10.1186/1471-2229-11-43Cite this article as: Keeling et al.: Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp. ). BMC Plant Biology 2011 ... spectra and retentionindices with authentic standards if available, and retention indices, and/ ormass spectra from Adams [52] and NIST, and combined mass spectra and retention index library searches...

Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... basalATPase activity and has chaperone activity in prevent-ing the aggregation of luciferase and reactivatingheat-inactivated luciferase. Deletion of the N-terminalconserved repeat I (amino ... USA) [34]. The deletion mutants of ClpC1 were also similarly expressed and puriﬁed.ATPase assayFor a standard assay, 5 lg protein was incubated in a 50 lL reaction mixture containing buffer A, ... Kar, Deepa Sikriwal*, Parthasarathi Rath*, Rakesh K. Choudhary and Janendra K. BatraImmunochemistry Laboratory, National Institute of Immunology, New Delhi, IndiaChaperone proteins are vital...

báo cáo khoa học: "Transcriptome analysis by GeneTrail revealed regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana" pdf

... 5.1) and made comparable by scaling theaverage overall signal intensity of all probe sets to a tar-get signal of 100 (Affymetrix GeneChip Operating soft-ware, GCOS) [15,16]. Data are available ... http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24348.Statistical analysis of microarray expression data and calculation of fold changesForfurtherdataanalysis,thedataextractedfromtheAffymetrix Microarray Suite Microarray ... categories in response toalterations of iron homeostasis in ArabidopsisthalianaMara Schuler1, Andreas Keller2, Christina Backes2, Katrin Philippar3, Hans-Peter Lenhof2 and Petra Bauer1*AbstractBackground:...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... these amino acids and amino acid derivatives, and values of DGD°, measured in triplicate, are given in Table 1.The effect of polyols on the kinetic parameters (Km and kcat) of the RNase -A mediated ... R. Singh*,, Tanveer A. Dar*,à, Vikas Rishi§ and Faizan AhmadCentre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, IndiaIntroductionBoth prokaryotic and ... Tm(midpoint of denaturation) and DHm(enthalpy change atTm) using a nonlinear least squares analysis according tothe relationship described earlier (see equation (1) in [25]).Using a value of...

Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt

... electron transport chain. Recent data in Arabidopsis indicatethat initialization of the molecular processes in both cases involve the activ-ity of the thylakoid membrane-associated kinase, STN7. ... by wind. Biotic in uences depend mainly on the individual position of a plant within an ecosystem, the density of the plantstand or, in case of algae, water depth and transpar-ency [3–6]. In ... in the alga Chla-mydomonas reinhardtii and in the higher plant Arabid-opsis thaliana, two orthologue thylakoid-associatedkinases (TAKs), called STT7 and STN7 [24,25], wereidentiﬁed and found...

Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... crustacean and insect hemocyanins. All analyses support a pancrustacean taxon of the insect and crustacean proteinsthat excludes the myriapod hemocyanins. Within the clade of myriapod hemocyanins, ... SDS/PAGE gel (Fig. 1).Two nonmatching amino acids at positions 3 (Glu instead of Cys) and 5 (Ala instead of Pro) may be explained by thepresence of an additional subunit in the sample that mostlikely ... regions. The standardpolyadenylation signals (AATAAA) and the poly (A) -tails of different lengths are present in each clone. The openreading frames of the ﬁve sequences translate into distinctpolypeptides...

Báo cáo khoa học: Val216 decides the substrate speciﬁcity of a-glucosidase in Saccharomyces cerevisiae doc

... chimera, a 27amino acid fragment of Mun/Bst including consensusFig. 1. Comparison of amino a cid sequencesbetween maltase and is oma ltase. Identical and similar amino acid resi dues are designated ... 574–2578.35. Nakao, M., N akayama, T., Kakudo, A. , Inohara, M., Harada,M., O mura, F. & Shibano, Y. (1994) Struc ture and expression of a gene coding for t hermostable a- glucosidase with a broad substratespeciﬁcity ... structuralfamilies [1,2]. Family 13 (a- amylase family) includesenzymes such as a- amylase, a- glucosidase, pullulanase,cyclodextrin glucanotransferase, and 1,4 -a- D-glucanbranching enzyme, speciﬁcally...

... graph of micro-average F -measureagainst the number of training sentences duringtext chunking (A: MHHMM, B: HHMM and C:HMM)The ﬁrst ﬁnding is that the size of training datadramatically affects ... is part of the Lancaster Treebank corpus and contains 1473 sentences. Each sentence con-tains hand-labeled syntactic roles for natural lan-guage text. A. 200 A. 400 A. 600 A. 800 A. 1000 A. 1200 A. 14000.860.880.900.920.94B.200B.400B.600B.800B.1000B.1200B.14000.860.880.900.920.940.860.880.900.920.94FC.200C.400C.600C.800C.1000C.1200C.14000.860.880.900.920.940.860.880.900.920.94FFigure ... model makes no use of the word infor-mation contained in the sentence.4.2 Grammar ParsingCreation of a parse tree involves describing lan-guage grammar in a tree representation, whereeach path...

Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinﬂammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf

... (forward, 5¢-AGGGTTGCC AGATGCAATAC-3¢;reverse, 5¢-ACACAGCTGGCAATGACAAG-3¢), MCP-1(forward, 5¢-GTGAGGAGCCACCAACATTT-3¢; reverse,5¢-GGGGGATCCCAAGTACTGTT-3¢) and GAPDH (for-ward, 5¢-CCCATCACCATCTTCCAGGA-3¢; ... attenuation in proinflammatory cyto-kine ⁄ chemokine production, indicating that the p38pathway acts predominately in the AGE-HSA and LPS amplified in ammatory response and, thus, mayserve as a main ... and aggravation of the disease.It is well established that proinflammatory media-tors initiate a cytokine⁄ chemokine storm by activatingthe intracellular signaling pathways, including MAPkinases...

Báo cáo khoa học: Protein dissection enhances the amyloidogenic properties of a-lactalbumin potx

... 65001020304050LATh1-LAdesβ-LApH 7.4Th1-LAdesβ-LAABCDFig. 2. Conformational characterization of Th1-LA and desb-LA by CD (A, B) and ANSbinding (C, D). Far-UV CD spectra of Th1-LA and desb-LA were ... proteolysis of bovine a- lactalbumin:Isolation and characterization of protein domains.Protein Sci 8, 2290–2303.32 Polverino de Laureto P, Vinante D, Scaramella E. FrareE & Fontana A (2001) ... that a minimization in acid solution of the negative charges of the carboxylates of the Asp and Glu residues in thecalcium-binding region of LA can produce a markeddecrease in the charge-to-charge...

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