... Department, AlbertEinstein College of Medicine, New York, USA, personalcommunication). Ten milliliters of C2HCl3were added to 11 mL of subunit c [1 mgÆmL )1 in chloroform/methanol(2 : 1)] and the ... C-terminal part (T67, G6 8) of the protein. Similar to the SDS sample, subunit c in chloroform/methanol/water(4 : 4 : 1) contains a nonhelical linker peptide near P28,which is shorter t han in ... without interrup-tion of the helical folding in t he hydrophilic loop region asindicated b y h elix-characteristic NOEs. Subunit c ofP. modestum therefore does not fold into a s table helicalhairpin...