... substrate discriminationagainst binding of the 5-OH group of Kdo and in favour of the N-acetyl group of Neu5Ac and, although these residues are conserved in CNSenzymes, none is conserved in the related ... important in the literature and haveroles assigned [12–18,20]: *, CTP-binding residue of the P-loop; (*), CTP-binding residue; ^, Neu5Ac-binding residue; #, Neu5Ac-bindingresidue forming part of the ... al. [14] in the related enzyme, L-CKS,because mutation would interfere with both Neu5Acbinding as well as playing a role in the re-orientation of Mg2+binding during the re-opening of the activesite...