báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx
báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx
... 11:117http://www.biomedcentral.com/1471-2229/11/117Page 11 of 14RESEARC H ARTIC LE Open AccessA single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters ... (R69L) located in the R2 domain of VvMYB5b and predicted to affect the formation of a salt bridge within the protein. The activity of the mutated protein (name VvMYB5b L, the native protein being ... as: Hichri et al.: A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity. BMC Plant Biology...
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx
... Comparison of the three pri-mary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas. Derivation of the amino acid sequence of ovine DNase and revision of the previously ... anesthesia withdiethyl ether. Preparation of the samples for the assays and determination of enzyme activity were performed asdescribed previously [7,21–22]. The N-terminal aa se-quences of the ... Cys173 and Cys209, which form the disulfide bond responsible for the stability of the enzyme[37], and also Arg41 and Tyr76, that mediate DNase I–DNA contact in the other vertebrate DNases I and orientate...
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Báo cáo y học: "A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5α" potx
... model of the capsid protein To obtain further insight into the effects of the G-to-E single amino acid substitution at the 116th position of the CA on its three-dimensional (3-D) structure, the ... Structural model of the N-terminal domain of HIV-1 CA with G116E substitution. Panel A shows the template structure of the N-terminal domain of the HIV-1 CA; panel B shows the model of the domain structure ... 2)was the result, at least partly, of the acquisition of the G116E substitution in the CA. The amino acid residue at the 116th position of the CA affects viral growth in the presence of TRIM5αWe...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... clearly demonstratethat the conformation of CD2 is unchanged. After the insertion of the helix–loop–helix EF-hand domain from STIM1, the helical content of the engineered protein CD2.STIM1.EF increased, ... are sensed by the canonicalEF-hand motif and cause conformational changes inthis motif. The Ca2+signal change and the accompa-nying conformational change in the canonical EF-handare probably ... ([Ca2+]ER) and resulted in constitutive punctae formation and Ca2+influx. In addition, the grafted single EF-hand motif formed a dimer regardless of the presence of Ca2+, which conforms to the EF-hand...
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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf
... half-reaction involving the conversion of FAD(flavin cofactor) to FADH2 and concomitant oxidation of the amino acid to an imino acid. The imino acid intermediate of the oxidation pathway undergoesnonenzymatic ... 390 and 475 nm, 375 and 450 nm, and 370 and 450 nm, respec-tively. The spectral features of the dissociated cofactorwere similar to those of FAD. The shifting of absorp-tion maxima of the ... anchoring at othersites in L1. In both enzymes, the carboxylate group of the inhibitors improved the affinity for the respectivesites. To determine the importance of the amino group for ligand anchoring,...
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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot
... obviates the need of looping of interveningDNA. The in vivo data supporting the MutS–MutLfoci formation suggests the possibility of extensiverecruitment of protein molecules at the sites of mis-match ... analysespresented here offers an equilibrium study of the com-plex changes brought about by MutS ATP hydrolysis and mismatch binding. Therefore the current studyaddresses the dynamic changes of the system ... mirrored the sedimentation prop-erty of the free protein observed in the earlier assaysuggesting that the DNA sedimentation accompaniedthat of protein. In order to verify whether all of the components...
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Báo cáo khóa học: A single mutation that causes phosphatidylglycerol deficiency impairs synthesis of photosystem II cores in Chlamydomonas reinhardtii pdf
... synthesis. In the absence of D1, the rate of synthesis of apoCP47 is strongly reduced, while the rates of synthesis of both D1 and apoCP47 drops when D1 cannotassemble within PSII complexes, for ... reinhardtii,thefattyacidC16:1(3t) could increase the efficiency of PG for PSII corebiogenesis in the situation of active synthesis determined by the high growth rate of this alga [46]. Altogether, theseobservations ... process of the cotranslationalinsertion of the D1 protein into the thylakoid membrane,then one could imagine that the absence of PG leads to adrop in translational elongation of the D1 protein. ...
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Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt
... three less thanthat of the B. subtilis enzyme. In the former enzyme the corresponding amino acids were cysteine, alanine and serine.Therefore, it is possible that the thermophilic enzyme is ... central intermediate in the de novo and salvagebiosynthesis of pyrimidine, purine and pyridine nucleotidesas well as in the biosynthesis of the amino acids histidine and tryptophan [1,2]. In addition, ... deduced amino acid sequence and shows t hat the initial methionine has been removed. Comparison of the nucleotide sequences of the insert o f p JM1 a nd the originalinsert of pHO219 (GenBank and...
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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx
... °C for 10 min; 35 cycles of 95 °C for 1 min, 48 °C for 1 min and 72 °C for 1 min; 72 °C for 9 min. The PCR products were subcloned into the T-vector and sequenced. The nucleotide sequence of the ... ‘+’. The asterisk indicates the specific band of the antibacterial protein. (C) 2D gel electrophore-sis shows a single spot of antibacterial protein indicated by a circle. The positions of the ... electrophoresis. Furthermore, we detected the Fig. 4. The cDNA and amino acid sequences of Platichthys stellatus antibacte-rial protein. The nucleotide sequence of cDNA encoding the PSEM antibacterialprotein...
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Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx
... both protein variants, the rate of reduction of the FMN cofactor was very small,amounting to 0.6% and 3% of the wild-type rate for the D137L and K109L ⁄ D137L protein variants,respectively. The ... against each other by 90° and packing of the two dimers. To test the hypothesis thattetramerization is responsible for increased thermosta-bility, the apparent melting temperatures of the protein variants ... performedto determine whether the reductive or the oxidativehalf reaction is impaired in the protein variants. The reductive half reaction of wild-type, the D137L and the K109L ⁄ D137L protein variant...
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