Báo cáo y học: "Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)" pdf
Báo cáo y học: "Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)" pdf
... pepstatinA had primarily inhibited the processing events.Functional role of cathepsin D/E in the processing of PPD by XS52 DCTo identify the protease(s) inhibited by pepstatin A, XS52DC were ... and VaccinesOpen AccessOriginal researchIdentification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)Mansour Mohamadzadeh*1, Hamid Mohamadzadeh2, ... activities. On the other hand, inhibitors of serine proteases (dichloroisocoumarin, DCI) or of cystein proteases (E-64) did not impair XS52 DC presentation of PPD, nor did they inhibit cathepsin D/E...
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Báo cáo y học: " Ethnoveterinary plant remedies used by Nu people in NW Yunnan of China" potx
... equally during the field work, data analysis andpreparation of the manuscript, and read and approved the final manuscript.Competing interests The authors declare that they have no competing interests.Figure ... [39]. The cost and availa bility of medicines and providers is a factor driving the use of alternative providers, including self-treatment using eth-noveterinary practices and purchasing medicines ... other communities in Gongshan County,disease was a major factor causing animal mortality andconstraining the development of animal husbandry [9]. The morbidity and death rates of animals in...
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Báo cáo y học: "Pro/con clinical debate: Are steroids useful in the management of patients with septic shock" ppt
... useful in the management of septic shockKatherine G Craig and Keith R Walley The debate surrounding the use of corticosteroids in septicpatients has continued for over 30 years. Two meta-analyses ... hasconflicting support in the literature. In this issue of Critical Care Forum, the two sides of this oftenheated debate are brought to the forefront in an interesting format.Keywords glucocorticoids, ... corticosteroids may actuallycause harm, as evidenced by a slight increase in overallmortality [1].Table 1Summary of prospective studies suggesting benefit for use of corticosteroids in patients...
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Báo cáo y học: "Pro/con clinical debate: Antibiotics are important in the management of patients with pancreatitis with evidence of pancreatic necrosis" ppt
... mortality. They included a study by Luiten and colleagues[8], which used selective decontamination of the digestivetract. Selective decontamination is attractive as it may allow the use of prophylaxis ... mortality rate, 0% versus 15%), butno difference in the rate of infection of pancreatic necrosis[4].Pooling of the data from these 183 patients by Bosscha andcolleagues in a meta-analysis resulted ... potential risk of the development of bacter-ial resistance.While there is inconclusive data supporting the use of pro-phylactic antibiotics in the setting of severe pancreatitis,there is some...
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Báo cáo y học: "Bench-to-bedside review: Hyperinsulinaemia/euglycaemia therapy in the management of overdose of calcium-channel blockers" ppt
... andincreasing the inotropic effect of insulin [21,24].Recommendations for the use of HIET in CCBpoisoningAlthough there is wide variation in insulin dosing in the casesreporting the use of HIET in ... Improvement of heart performanceinduced by low-dose insulin was not accompanied by anincrease in myocardial oxygen consumption. Myocardialuptake of glucose increased significantly, whereas uptake of lactate ... supported by cardiopulmonarybypass, high-dose insulin (aortic root bolus of 1,000 IUinsulin, with a glucose clamp maintained at physiologicallevels) reversed the negative inotropic effect of propranolol(0.2...
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Báo cáo y học: " HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain" doc
... [30]. In the current study, the M4 mutant wasstudied to clarifying why oligomer formation is essentialfor Rev activity by assessing the requirements for restora-tion of the activity of the mutant.Results The ... Rex. The function of the HTLV-I Rex protein is similar to that of Rev and the basic domains from the two viral proteinsbind to Importin β during nuclear import [35,36]. It istherefore likely that ... corresponding to the basic domain of Rev inhibited the in vitro splicing of RRE containing mRNAs underscores the functional importance of this region [39,40]. Theseresults therefore support the suggestion...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
... presented by coefficient of variance (CV). CVs are calculated by dividing the standard deviation (SD) by the mean ratio of a gene and multiplying by 100%. The minimum and maximum numbers of measurements ... the Cyber-T implementation of a variant of the t-test [31]. False discovery rates (FDRs) were calculated by (i) ranking the genes by p-value, (ii) multiplying the p-values with the number of ... preference of the reverse transcriptase enzyme for the Cy3 label and (ii) prolonged exposure to air and light of the dyes increasing the chance of oxidation and / or bleaching. The main contributing...
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Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"
... that bind to the SH2 domains. The tandem SH2 domains bind to Tyr-627 and Tyr-659 simultaneously in a specific orientation, in which Tyr-627 binds to the N-SH2 domain and Tyr-659 binds to the ... previously that G/G of the PTPN11 gene encoding SHP-2 increased the risk of gastric atrophy among the seropositive subjects [18]. The present study examined the association of a polymorphism of ... diagnosis of cancer. In summary, the Gab1 A/A was associated with the low risk of H. pylori infection while the G/A and A/A genotypes together may increase the risk for gastric atrophy. The biological...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... amino-acidcomposition of hyperthermophilic proteins is the biasagainst thermally labile amino-acid residues. This pattern isobvious on examination of the amino-acid compositions of the total protein content of ... increasefrom the apoenzyme to the Mn2þ-containing enzyme, loss of the metal cofactor could be the limiting step in BLXIinactivation. Higher Eavalues of inactivation for the Co2þ–enzyme and the ... on xylose, the apoenzyme activity was tested on both sugars in the presence of increasing concentrations of each cation (in the chloride form; Fig. 4). In the absence of metal, the apoenzyme...
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Báo cáo Y học: Two 1 : 1 binding modes for distamycin in the minor groove of d(GGCCAATTGG) docx
... asoutcomes of such calculations may be spoiled by inaccuratepositioning of the interacting groups. The contact area of the distamycin amidinium end wasinvestigated by evaluating the hydrogen positions ... between the pyrrole rings form bifurcated hydrogen bonds with the thymine or adenine bases. The central N5(D) atom of the drug is hydrogen-bonded to the two O2 atoms of the thymine bases of the central ... AT-rich sequences [1],thereby preventing DNA and RNA synthesis by inhibition of the corresponding polymerase reaction. The crystalstructure determination of a 1 : 1 distamycin–d(CGCAAATTTGCG)...
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