... USA).Activity assay The activity of SOD was assayed with a SOD Assay Kit-WST (Dojin, Kumamoto, Japan) at 37 °C via the xanthineoxidase-WST-1 method [34]. In accordance with the manufacturer’s instructions, ... Kodai Machida3,4,Atsushi Suzuki5and Yasushi Kawata3,41 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan2 Department of Food Science and Nutrition, Faculty ... ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical...