... per-meabilization step was omitted. Cells were stained with a mouse IL2RA antibody followed by an Alexa 488-conju-gated goat secondary antibody.Western blot analysis For analysis of the total wt and ... IL2RA, and rat Mycantibodies were purchased from Stressgen (Ann Arbor, MI,USA), Zymed Laboratories (South San Francisco, CA,USA), BD Biosciences (San Diego, CA, USA), Upstate(Lake Placid, ... exhibits a steady-state localizationin the Golgi apparatus and an unknown vesicle com-partment in the stably transfected CHO cells and the disruption of a di-leucine signal in the variable loopregion...