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http://www.jnanobiotechnology.com/content/9/1/27
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RESEA R C H Open Access
Atomic Force Microscope nanolithography on
chromosomes to generate single-cell genetic
probes
Sebastiano Di Bucchianico
1*
, ... regions, identified
by topographic banding, to perform the nanodissection
experiments. Upon localization of the chromosome
region to be dissected the AF...
... differentiation of hMSCs, we used the combination
of AZA (an inhibitor of DNA methylation) and TSA (an
inhibitor of histone deacetylases) prior to start of cardiac
differentiation protocols in vitro. ... hypermethylation and chromatin
compaction by histone deacetylation are proved to be
important mechanisms by which some gene expression
can be silenced in stem cells [18,19]. To push the...
... fixation like in elec-
tron microscopy. Conventional microscopy not only dis-
torts sperm morphology, but is also unable to provide
high-resolution 3D images owing to the small size of the
spermatozoa. ... axoneme region in
addition to depressions of the membrane that could not
be observed with the conventional microscope has been
reported. The 3D image contrast mechanism has been uti...
... 89–96 ª 2007 The Authors Journal compilation ª 2007 FEBS 89
found to be prone to aggregation and precipitation at
high concentration. By contrast, a longer Mb-derived
fragment, spanning residues ... simulations were performed at 400 K. During the
simulations, the non-bonded interaction cut-off was set to
12 A
˚
, which is sufficiently large to include long-range elec-
trostatic interac...
... personalised oral communication with
smokers, but none on personalised written com-
munication, because no one currently writes per-
sonalised letters to smokers). In such applica-
tions, the only ... STOP letter generated from the Figure 1 questionnaire
Information for Stopping Smoking
Do you want to stop smoking?
Everyone has things they like and dislike about their smoking. The
decis...
... phosphoglycerol
modification of eukaryotic elongation factor 1A [44].
It is worth mentioning that the dependence of GPI
and ethanolamine phosphoglycerol synthesis on PE as
the ethanolamine donor was demonstrated ... been
reported, one localizing to the ER and one to the Golgi [89]. The
corresponding model proposes that the ER enzyme preferentially
uses inositol formed de novo from glucos...
... phosphate solution (pH 6.0).
Preparation of tag-free hEx3 diabodies from the
Fc fusion format
To construct the expression vector for preparing tag-free
diabodies by using IgG-like BsAbs, we connected ... was
dissolved to saturation in water:acetonitrile (2 : 1 v ⁄ v) con-
taining 0.067% trifluoroacetic acid. Sample solutions
from each stage were mixed with the sinapic acid-saturated
soluti...
... ‘tropical storms’, retrieving a document
only containing tropical can be meaningless, but a
document about storm can be meaningful. Based
on this observation, we only incorporate document
frequency ... are
potentially useful for not only discriminating a
phrase itself but characterizing its constituents. In
this section, we report only the ones that have
made positive contributions to the...
... normalized
10-bin histograms for the 7 features were concate-
nated to result in a single 70-dimensional obser-
vation vector for the turn. These 70 dimensional
observation vectors for each turn ... accommodation along a decreolizing contin-
uum. International Journal of the Sociology of Lan-
guage, 1984(46), 71–86.
Putman, W. & Street Jr, R. (1984). The conception
and perception of non...
... positions. The latter
residue has a gauche conformation along the C
b
–C
c
bond (torsion angle C
a
–C
b
–C
c
–S
d
of 50.0°, 48.2° and
50.4° in molecules A, B and C, respectively). As in
other cytochrome ... is adjacent to the second
heme ligand, Met65, and Pro66 undergoes conforma-
tional changes upon oxidation ⁄ reduction [16]. Whereas
in cyanobacterial, plant and red and brown algal cyto-...