... j chains andeach HuVkFOR with a mixture of HuJkBACK for kchains. A GeneAmp PCR thermocycler (Perkin-Elmer9600, Norwalk, CT, USA) was used for PCR. The condi-tions for the amplifications were: ... determine the corresponding germ lines. For clone 3F, VH3-VK3 were the closest families for VHand VLdomains, respectively. In the case of C1, VH3-Vk1 were the families with highest scores. The ... mixture of HuJHBACK primers [40] in inde-pendent reactions for each family. For light chain variabledomains, a similar procedure was performed using eachHuVjFOR and a mixture of HuJjBACK for j...