báo cáo khoa học: "Differential Expression of MicroRNAs in CD34+ Cells of 5q- Syndrome" pdf

... leads to the 5q- like p he-notype [20]. In our study, miR-146a expression wasdown-regulated by 30% in BM CD34+ cells of 5q- patients. Interestingly, gene expression of this miRNAshowed increased ... withthese findings, Straczynowski et al. detected significantdown-regulation of miR-143 and miR-145 in 5q- marrow cells and down-regulation of miR-145 and miR-146a in CD34+ cells of three 5q- patients ... al. Journal of Hematology & Oncology 2011, 4:1http://www.jhoonline.org/content/4/1/1Page 6 of 6SHOR T REPOR T Open AccessDifferential Expression of MicroRNAs in CD34+ Cells of 5q- SyndromeHana...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... Molecu-lar cloning and cold-inducible gene expression of ferritinH subunit isoforms in rainbow trout cells. J Biol Chem271, 26908–26913.13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & ChuangMJ ... pattern of proportional pooling of subunitswith different kinetic and thermodynamic properties of the tetrameric enzymemay result in ﬁne-tuning of the properties of skeletal LDH-A, which is in linewith ... insert in the 3¢-UTR of mRNAbldhÀa(Fig. 2), this fact excludesthat these are products of alternative splicing of thesame transcript. In contrast, it points directly to theexistence of two independently...

Báo cáo khoa học: Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages potx

... crosseda threshold in the log-linear range of RT-PCR, indicatingthe relative amount of starting template in each sample.The fold change in expression of Neu1, Neu3, and Neu4RNAs in macrophages ... consequentdesialylation of surface glycoconjugates in activated cells induced production of interleukin-4 by lympho-cytes [2], enhanced binding of CD44 on the surface of monocytes to hyaluronic acid, a component of ... acid bindingIg-like lectins (siglecs) on their surface [reviewed in 44].As some of these siglecs have binding sites that aremasked by sialic acid on resting cells, it is possiblethat during...

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... & Hunter T (1995) Howdo protein kinases discriminate between serine ⁄ threo-nine and tyrosine? Structural insights from insulin recep-tor protein-tyrosine kinase. FASEB J 9, 1255–1266.Supplementary ... germinal epithelium or in spermatozoa, in cells from the interstitial tissue, Leydig cells, vascular cells or myoid cells in the lamina propria.Stk33-speciﬁc ISH signal were also detected in ... in line withthe strong immunostaining signal in mouse embryoACDBFig. 3. Stk33 recombinant protein, antibody and spermatogenesisspeciﬁc signal in mice. Western blot of recombinant biotin–Stk33fusion...

Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf

... overexpression of enolase 1and malic enzyme 1 supports the induction of fattyacid metabolism. The reduction in d-amino acid oxi-dase 1 expression in the kidney proteome is interesting, in view of the ... Neuhoff V, Arold N, Taube D & Ehrhardt W (1988)Improved staining of proteins in polyacrylamide gelsincluding isoelectric focusing gels with clear backgroundat nanogram sensitivity using ... Compara-tive analysis of protein spots was performed by matchingcorresponding spots across different gels. Each of thematched protein spots was rechecked manually. Intensityvolumes of individual spots...

báo cáo khoa học: " Differential expression of cysteine desulfurases in soybean" potx

... an aminotransferase class-Vmotif and alignment analysis showed the location of acysteineintheactivesiteandahistidineandalanineinthe cofactor binding site (Additional files 1 and 2). Tofind ... proteins of the [Fe-S]cluster biosynthesis pathway, i.e. altering the expression of cysteine desulfurase genes. Stress dependent changes in gene expression occur in the cytoplasm a s well as in chloroplasts. ... responsive elementLS7 x part of a light responsive elementMBS x (2) x (2) x (1) x (1) MYB binding site involved in drought-inducibilityMBSI x MYB binding site involved in flavonoid biosynthetic...

Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

... 10’17β-HSD1117β-HSD11L-FABPL-FABPCFig. 2. Expression of 17 b-HSD11 protein in the gastrointestinaltract. (A) The gastrointestinal tract of mice [29] fed a diet containingWy-14 643 for 5 days was divided into 10 parts, ... blot50CBB-stain3725IntestineLiver+ ++ +Wy14,64317β-HSD11CBB-stainBAFig. 1. Effects of Wy14 643 on protein expression levels of 17b-HSD11 in the liver and intestine of mice. (A) Speciﬁcity of theantibodies ... 17b-HSD11 was greatly induced in the intes-tine and liver of mice by administration of a PPARaagonist, Wy-14 643 [5], the changes in protein levelswere examined by western blotting. For this purpose,we...

Báo cáo khoa học: Novel suppression mechanism operating in early phase of adipogenesis by positive feedback loop for enhancement of cyclooxygenase-2 expression through prostaglandin F2a receptor mediated activation of MEK⁄ ERK-CREB cascade doc

... Involvement of MEK signaling in the enhancement of COX-2 expression. (A)Inhibition of Fluprostenol-activated COX-2 expression by kinase and COX inhibitors.3T3-L1 cells were cultured in the presence of ... CREB is also involved in the regulation of COX-2 gene expression in various cells [43] through activation of protein kinase C andCa2+signaling cascades in ileal epithelial IEC-18 cells [44] ... the promoter region of the COX-2 gene in 3T3-L1 cells. These results indicate that PGF2asuppresses the progression of the earlyphase of adipogenesis by enhancing the binding of CREB to the COX-2...

Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

... a notable 30- to 40-fold increase in cGMP levelsduring a 30-min incubation (Fig. 2); indeed a doubling of cGMP levels could be observed within 2 min of application of hormone (results not shown). ... Neuropeptide-induced inhibition of steroidogenesis in crab moulting glands: involvement of cGMP-dependent proteinkinase. Gen. Comp. Endocrinol. 104, 41–51.20. Sedlmeier, D. & Seinche, A. (1998) ... suggested.Levels of MIH and CHH in the sinus gland duringthe moult cycleAs individual SG from single crabs always contained almostidentical proﬁles and quantities of peptides (preliminaryexperiments using...

Báo cáo khoa học: Adipocyte hyperplasia and RMI1 in the treatment of obesity doc

... suggestingthat these cyclin-dependent kinase inhibitors areimportant in the regulation of adipocyte number.The S-phase kinase-associated protein (Skp)1–Cullin-F-box protein (SCF) ubiquitin ligase ... showna reduced number of b cells in the pancreas [14]. In addition, overexpression of p27Kip1 in b cells induced hyperglycemia in mice as a result of inhibition of b-cell proliferation [18]. These ... escalating prevalence of obesity is one of the most pressing healthconcerns of the modern era, yet existing medicines to combat this globalpandemic are disappointingly limited in terms of safety...

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