... Japan, Tokyo, Japan), and stained withanti-spHAS antibody according to the method of Towbinet al.[35]using3,3¢-diaminobenzidine tetrahydrochloride(Dojindo Laboratories, Kumamoto, Japan) for ... 25–1190 of AF023876.RNA preparations and Northern hybridizationTotal RNA was prepared from FM100 cells using a RNeasy Plant Mini Kit (Qiagen Japan, Tokyo, Japan)according to the manufacturer’s ... Shinomura, T., Hamaguchi, M., Yoshida, Y. ,Ohnuki, Y. , Miyauchi, S., Spicer, A. P., McDonald, J .A. &Kimata, K. (1999) Three isoforms of mammalian hyaluronansynthases have distinct enzymatic...