... results). The enzyme activity of the recombinant LDH was assayed at 70 ° C by monitoring the lactate-dependent NADH oxidation as the decrease in A 340. The standard assay mixture contained 1 mm lactate,0.2 ... acetyl-CoA was incubated at70 °C under an argon atmosphere. The reaction was startedby adding the NADH, acetyl-CoA and enzyme solutions to the mixture, and the decrease in A 340as a result of NADHoxidation ... Ozawa Y, Nakamura T, Kamata N, Yasujima D,Urushiyama A, Yamakura F, Ohmori D & Imai T(2005) Thermococcus profundus 2-ketoisovalerateferredoxin oxidoreductase, a key enzyme in the archaealenergy-producing...