... USA) [34]. The deletion mutants of ClpC1 were also similarly expressed and purified.ATPase assayFor a standard assay, 5 lg protein was incubated in a 50 lL reaction mixture containing buffer A, ... chaperone activity in prevent-ing the aggregation of luciferase and reactivatingheat-inactivated luciferase. Deletion of the N-terminalconserved repeat I (amino acids 1–38) resulted in analteration ... Kar, Deepa Sikriwal*, Parthasarathi Rath*, Rakesh K. Choudhary and Janendra K. BatraImmunochemistry Laboratory, National Institute of Immunology, New Delhi, IndiaChaperone proteins are vital...