... When anti-Sp1 IgG was used, complexC1 disappeared and a new complex, SC1, with a highermolecular mass was formed (lane 11), and, when anti-Sp3IgG was used, bands C2 and C3 disappeared and bandsSC 3a ... bandsSC 3a and SC3b appeared (lane 12). Coaddition of the twoantibodies resulted in the loss of bands C1, C2, and C3 (lane13). In contrast, the use of a control monoclonal antibodyagainst SREBP ... detected. The intensity of the 105 kDa band was significantlyincreased in the PMA treated cells than in the control cells,whereas that of the 95 kDa band was not changed(Fig. 5A) . Using anti-Sp3...