... tray.The tray was then placed on top of a hot plate and thetemperature maintained at 9 0 °C for 5 min to aid thestaining of protein spots. The tray was then placed on a laboratory shaker for a further ... Ohkura, M., Furukawa, K ., F ujimori, H., Kuruma, A. ,Kawano, S., Hiraoka, M., Kuniyasu, A. , Nakayama, H. &Ohizumi, Y. (1998) Dual regulation of the skeletal muscle rya-nodine receptor by ... 211–218.40. Maeda, T., Chapman, D.L. & Stewart, A. F. (2002) Mammalianvestigial-like 2, a cofactor of TEF-1 and MEF2 transcriptionfactors t hat promotes skeletal muscle d ifferentiation. J....