báo cáo khoa học: " Statin-induced apoptosis via the suppression of ERK1/2 and Akt activation by inhibition of the geranylgeranyl-pyrophosphate biosynthesis in glioblastoma" potx
... fluvasta-
tin and simvastatin decrease the activation of ERK1/2
Akt [4]. These findings are in agreement with the results
of the present study and indicate that statins induce
apoptosis via suppression ... whether the inhibi-
tion of ERK1/2 and Akt activation in C6 glioma cells
was due to the inhibitory action of statins on FPP or
GGPP biosynthes...
... Animal
Institute of Peking Union Medical College and maintained
under standard conditions according to the guidelines of
the Institutional Animal Care and Use Committee of
Peking University. The ... cell
growth by inducing apoptosis [21] and inhibits the migra-
tion, invasion a nd adhesion capacity by down-regulating
the expression of CD44
v6
protein [22]. Howeve...
... (min)
pAkt /Akt (R.U.)
AKT
pAKT
03060
Time (min)
BBG 100 nM
AB
CD
EF
Fig. 8. P2X7 receptor inhibition increases
Akt and GSK3 phosphorylation in N2a cells.
(A, B) Western blots of p
S473
Akt and ... resulting in Akt activation and GSK3
inhibition. It should be noted that increased Akt activ-
ity can also induce the inhibition of GSK3, which has
been proposed t...
... group of
CDK inhibitors comprising two families of proteins:
inhibitor of kinase 4 ⁄ alternative reading frame and
CDK inhibitor protein ⁄ kinase inhibitor protein. The
inhibitor of kinase 4 ⁄ alternative ... kinase inhibitor protein family includes
p21
WAF1
, p27
Kip1
, and p57
Kip2
. They halt the cell cycle
in the G
1
phase by binding to, and inactivating,
cyclin–CD...
... Cd
2+
on the
kinetics of the CoQ-oxidizing module (A) and
CoQ-reducing module (B). Effect of Cu
2+
on
the kinetics of the CoQ-oxidizing module (C)
and CoQ-reducing module (D). The kinetics
of the CoQ-oxidizing ... CoQ-oxidizing module were obtained
by titrating with a specific inhibitor of
the CoQ-reducing module, malonate
(0–3.125 l
M). The kinetics of the...
... All-trans retinoic acid inhibits KIT activity
and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell
line by affecting on the expression of survivin and Bax protein. Journal
of Experimental ... and imatinib
The result of isobologram was showed in Figure 5B. All
data points in the combination fell within the envelope
of additivity, the area surro...
... of the input and the eluate of the chromatography
were resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo-
massie Brilliant Blue staining (left) and western blotting (right).
Lane 1: input ... suggesting that this peptide may also
affect the development of the wing disks at the prepu-
pal stages. It is interesting to note that bombyxin-II
also stimulated th...
... that the most characteristic signatures in the
sequences are present in both proteins, tyrosinases and
catechol oxidases; these include the six histidine resi-
dues involved in the binding of a ... browning of fruits and vegetables, and
morphogenesis and fruiting body formation in fungi.
All of these processes involve melanin formation. In
the bacterial kingd...
... especially with the series of Mes-
sage Understanding Conferences of the nineties,
has focused on tasks such as creating and updating
databases of corporate join ventures or terrorist
and guerrilla ... on the pro-
blem of identifying and organizing into a useful
database structure the different linguistic consti-
tuents of the candidate predications, a phase simi-...
... monitored by the reduction of
cytochrome c in the presence of NADH at 550 nm [1].
Protein determination
Protein content was determined by the Bradford test [20],
using bovine serum albumin as standard.
PHI ... explain the regulatory effect. In fact, on addition of PHI,
the fluorescence of PHR-bound flavin increases. This
finding points to a PHI-induced conformational ch...