... purposes)
Journal of Circadian Rhythms
Open Access
Research
Altered expression of circadian clock gene, mPer1, in mouse brain
and kidney under morphine dependence and withdrawal
Xiaojia Wang
1,2
, Yueqi ... mPER1 protein expressed in the brains and kidneys of Con, MD and MW miceFigure 4
Circadian variation of mPER1 protein expressed in the brain...
... limitation of video recording during
darkness by using a camera that, by automatically switch-
ing to infrared in complete darkness, allowed us to contin-
uously monitor activity during the light and ... the amount of overall
Activity of a rat in L:D and D:D conditionsFigure 6
Activity of a rat in L:D and D:D conditions. Top: 3rd day in LD conditions ; bottom: 5th day u...
... changes in
HAAP include marked proliferation of synoviocytes in the
synovial lining layer, gross infiltration of lymphocytes, and
migration of atypical lymphocytes with nuclear indentation
into ... osteophytes and definite joint space narrowing.
b
Scoring was as follows: +/-, minimum staining in one area of the
tissue; +, patchy staining involving several areas; ++, modera...
... chronically inflamed synovial tissue [7,8].
Both the residual synovial-lining cells and infiltrating leuko-
cytes are the source of pro-inflammatory chemokines such as
CCL2, CCL3, CCL5, CCL20, CXCL9 and ... were analyzed.
Expression levels of chemokine receptors were measured by
flow cytometry and were compared between the different
patient groups and healthy individuals. The an...
... by a variety of extracellular stimuli [1]. FAK and Pyk2
associate with the cytoskeleton and with integrin-signaling
complexes by binding to Src kinase and paxillin [2-5]. FAK is
rapidly tyrosine ... the
colocalization and activation of FAK, Pyk2, Src and paxillin in
RA and OA patient's ST lining and sublining may be important
for integrin-mediated signaling.
pPLCγ ex...
... co-localized in a great majority of cell
nuclei. Not only do these findings challenge the dogma of
mutually exclusive var gene transcription, they also add com-
plexity to the understanding of the ... thorough
insight into the field of genetic differences and the mecha-
nisms behind these could generate a better understanding of
the biology of P. falciparum, as well as of th...
... majority of deaths and can only be detected
noninvasively by complex diagnostics [4,5]. In contrast,
the underlying cause in older athletes who die suddenly is
usually atherosclerosis of the ... coronary artery anomaly with a left-side origin of the right coronary arteryFigure 2
Multislice computed tomography of the heart dem-
onstrates the coronary artery anomaly with a left-
s...
... Germany). Three differ-
ent fractions were collected in the order: substrate (cytidine
coeluting with nonreacted CDP), product (deoxycytidine)
and by-product (cytosine) for analysis by liquid scintillation
counting ... selection of transformants.
Protein techniques
Protein was determined by protein-dye binding with BSA as
a standard [47]. Whole cell protein of C. ammoniagenes cells
wa...
... FEBS 155
Determination of phosphatidylserine expression
and cell death
Phosphatidylserine expression on the outer part of the
plasma membrane was examined by Annexin V binding.
Brie y, cells were ... study, Nor3.2-reactive CD1d
has been found altered in keratinocytes from psoriatic
lesions [47], in the gastrointestinal tract of certain
in ammatory diseases [48,49], and in...
... (1997) The crystal structures of Sinapis alba myro-
sinase and a covalent glycosyl±enzyme intermediate provide
insights into the substrate recogn ition and active-site machinery of
an S-glycosidase. ... 2002
20 lL of the enzyme/inhibitor mixture to 180 lLof
substrate (4NPGlc), incubating for 30 min at 37 °C, and
measuring the rel ease of 4NP.
Protein assays and protein sequenc...