... housed
individually in standard breeding cages.
Methods
Electronic system for the recording of locomotor activity
The locomotor activity of the animal is recorded automat-
ically by means of microwave radar based ... very long
monitoring of the animals, creating continuous time
series. The data files are automatically saved to the hard
disk, allowing im...
...
Germany. They represented a convenience sample of
patients in that each was already scheduled for
coronary angiography for any indication and had no
history of a coronary revascularization ...
260
and economic evaluation, of myocardial perfusion scintigraphy
for the diagnosis and management of angina and myocardial
infarction. Health Technol Assess. 2004; 8: 1-207....
... Asia, and North America on
individuals of varying ages and degrees of disease
state including normal populations [20,21]. All ECG
ana
lyses in this database have been validated against
the ...
52
generate indexes of abnormality. The resulting
patterns of the indexes are then compared for
abnormality to the patterns in the reference database to
reach a final di...
... before analyzed by SDS-PAGE and
quantitated by using the BCA Protein Assay Kit (Be-
yotime, Shanghai, China).
2.4 Immunizations
Five-week old female BALB/c mice initially re-
ceived subcutaneous ... aspecific antibodies
that bind both the NPM-wt and NPM-mA proteins. In
IHC assay labeling with this kind of mAbs, the cyto-
plasmic subcellular localization of NPM1 may not be
cl...
... such as the
following: Do plasma sRAGE levels vary from day to day in a
subject? Do they vary over the lifespan of the individual?
What were the levels of sRAGE in the RA subjects before the
onset ... of sRAGE, at least in the synovial
fluid, might be modified by intense anti-inflammatory therapy.
Indeed, an emerging theme in the biology of RAGE links this
re...
... apical staining of this marker was
apparent in distinct areas of 3-D Huh7 aggregates (Fig. 3).
Taken together, these data demonstrate that the expres-
sion and distribution of cell adhesion and ... [2].
RNA isolation and RTqPCR
Total cellular RNA was isolated by the guanidine thiocy-
anate method using standard protocols [29]. One μg of
RNA was used for cDNA synthesis using Ta...
... that localizes to the apical plane of
polarized cells [45], was non-detectable in 2-D Huh7
monolayers, while, apical staining of this marker was
apparent in distinct areas of 3-D Huh7 aggregates ... both in 2-D and 3-D
Huh7 cultures; however, there was a profound decrease in
the accumulation of nuclear β-Catenin-containing com-
plexes in the 3-D Huh7 aggregates. Beca...
... a review of
clinical features, imaging and pathology. Neurotoxicology 1999,
20(2–3):227-238.
4. Takazawa K, Ishikawa N, Miyagawa H, Yamamoto T, Hariya A, Dohi
S: Metal allergy to stainless steel ... in the manage-
ment of the patient, writing of the manuscript and review-
ing of the literature. All authors read and approved the
final manuscript.
Consent
Written informed...
... light sedation) in accordance with the
COMFORT classification.
Data are expressed as mean ± standard deviation in the case
of a normal distribution and interval scaling level, and as
median (range) ... Berlin, Berlin, Germany
2
Professor of Cardiac Surgery, Department of Pediatrics, Campus Benjamin Franklin, Charité University Hospital Berlin, Berlin, Germany
3
Professor of...
... catalyzed by malic enzyme, pyruvate
phosphate dikinase, and phosphoenolpyruvate
carboxykinase.
In order to investigate the value of the model as a hypothesis
generating tool, we analyzed the in ... of a
commercial assay kit that employs a glycerokinase-coupled
enzyme assay system (Boehringer Mannheim, Mannheim,
Germany).
Assay of isocitrate lyase activity
Crude enzym...