Molecular characterization of the PhoPQ-PmrDPmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43 ppt
Molecular characterization of the PhoPQ-PmrDPmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43 ppt
... manner.Analysis of < /b> EMSA indicates a direct binding of < /b> the< /b> recombinant PhoP topmrD The < /b> binding of < /b> PhoP or Pm rA to PpmrHhas been deter-mined recently [31]. In order to determine whetherPhoP binds directly ... in F igure 5A, binding of < /b> the < /b> recombinant His-PhoP protein to PpmrDwas evident by the < /b> formation of< /b> a protein/DNA complex with a slower mobility. The< /b> binding specificity was also examined by ... deleting effect of < /b> pmrF in polymyxin < /b> B resistance was observed, and the < /b> survivalrate could be restored by the < /b> introduction of < /b> pR K415-PmrF. The < /b> results indicated a role of < /b> PmrF in the < /b> poly-myxin...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... almost continuous densityfor the < /b> backbone of < /b> CBH IB. The < /b> final model, 1Q9H,included 430 of < /b> the < /b> 437 amino acid residues of < /b> CBH IB. The< /b> final two amino acids and the < /b> loop region (from amino acids193–197) ... perhapsindicative of < /b> its flexibility. It is worth noting that the< /b> structures of < /b> T. re esei GH7 CBHs were solved in the < /b> presence of < /b> substrates. However, as 1Q9H was solved in the < /b> absence of< /b> bound substrate, ... deletion of < /b> these three residues, thus leading to a more open substrate-binding site.Catalytic binding siteBrooks et al . showed, by NMR, that the < /b> CBHs I fromT. emersonii has a retaining mechanism...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... by the < /b> Scientific ServicesDepartment of < /b> the < /b> Weizmann Institute by a single injection of < /b> 150 lg of < /b> the < /b> purified protein, followed by two boostershots of < /b> the < /b> same amount of < /b> protein at 2-week intervals.Serum ... AlexanderGirshovich who initiated and was actively involved in the < /b> beginning of< /b> this work. We wish to thank I. Gokhman for her help during the< /b> subcloning of < /b> YbdQ. This research was supported by the < /b> MINERVAFoundation, ... assumption needs to be examined further. In this regard, the < /b> exact mode of < /b> action of < /b> UspA, as well as that of < /b> other members of < /b> the < /b> UspA family remains to be explored.According to our findings, it is...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... polymeraseactivity on the < /b> temperature was determined by assaying the < /b> enzyme in the < /b> standard reaction mixture at the < /b> indicated temperatures. (D)Thermal stability of < /b> Rep245 was tested by pre-incubating the < /b> enzyme ... notgoverned by the < /b> sequence of < /b> the < /b> substrates used forthese assays was further supported by the < /b> finding thatRep245, when incubated with each of < /b> the < /b> above DNAoligonucleotides, proved able to incorporate ... °C between 190 and260 nm with a step increase of < /b> 0.2 nm, and a bandwidth of< /b> 1 nm. Thermal stability of < /b> Rep245 was measured by incu-bating the < /b> protein at 60, 70 and 80 °C for 30 min and thenrecording...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... characteristics of < /b> the < /b> lac1 proteinsuggest the < /b> absence of < /b> a type 1 copper site, this is not borneout by analysis of < /b> the < /b> deduced amino acid sequence of < /b> the< /b> enzyme. Thus, the < /b> 10 histidines and one cysteine ... geneexpression but also positively affected the < /b> activity andstability of < /b> the < /b> enzyme [40]. The < /b> effect of < /b> copper onenzyme stability may be related to the < /b> inhibitory effect of< /b> the < /b> metal on the < /b> activity of < /b> ... J. Biochem. 271) Ó FEBS 2003N-terminal sequencing of < /b> laccase The < /b> N-terminal amino acid sequence of < /b> purified laccase wasdetermined by electroblotting the < /b> enzyme on to an Immo-bilon poly(vinylidene...
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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot
... of < /b> OSBP proteins in sterol and sphingo-lipid metabolisms. The < /b> canonical OSBP is believedto play a role in regulating < /b> sterol biosynthesis,KeywordsOSBP; OSH; Osh6p; oxysterol-bindingprotein; ... N-terminal half containing the< /b> conserved ORD domain mediates lipid binding. Aweak binding to PtdIns(3)P by all proteins was alsoobserved, which might be nonspecific. Characterization < /b> of < /b> the < /b> cellular ... hydrolase-carboxypeptidase S. MVB is a pro-cess whereby the < /b> limiting membrane of < /b> late endosomesinvaginates and buds into the < /b> lumen of < /b> the < /b> organelle,and is responsible for the < /b> biosynthetic delivery of < /b> lyso-somal...
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Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx
... hydrolysis of < /b> bovine Hb.Bovine Hb (1 lg) was incubated withrHlSCP1 in 25 mM citrate ⁄ 50 mM phosphatebuffer (pH 4–7) or 50 mM phosphate buffer(pH 8). Co, reaction buffer containing bovineHb without ... initiallycleaves in the < /b> conserved hinge region of < /b> the < /b> Hb alphachains [49,50]. Falcipain, a member of < /b> the < /b> cysteineprotease family, degrades the < /b> denatured globin [50,51],and the < /b> resultant globin peptides ... exopeptidase involved in degradingvitellogenin [13]. The < /b> host’s blood-meal is the < /b> only source of < /b> energy in ticks. Unlike blood-sucking insects, ticks make a bloodpool by rupturing blood vessels under the...
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Báo cáo khoa học: Characterization of the molten globule state of retinol-binding protein using a molecular dynamics simulation approach ppt
... here. The < /b> binding of < /b> TTR toRBP has been shown to stabilize the < /b> binding of < /b> retinolto RBP. Studies of < /b> the < /b> interactions of < /b> chimaera of < /b> RBP and epididymal retinoic acid binding pro-tein (ERABP) ... RBP in the < /b> core of < /b> the < /b> b- barrel.When bound, retinol is almost totally encapsulated by the < /b> protein, the < /b> b- ionone ring lying in the < /b> centre of < /b> the< /b> protein with the < /b> isoprene chromophore stretchingalong ... in the< /b> native state, and which retains the < /b> majority of < /b> this b- sheet secondary structure in the < /b> molten globule state,human serum retinol-binding protein (RBP).RBP is a member of < /b> the < /b> lipocalin...
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Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... protein in rat brain In order to clarify the < /b> localization of < /b> the < /b> 105-kDa protein in the < /b> brain, the < /b> rat brain was dissected into six sections andTable 1. Properties of < /b> the < /b> 105-kDa protein, APG-1, ... sequence of < /b> the < /b> 105-kDa proteinTo investigate the < /b> biochemical properties of < /b> the < /b> 105-kDaprotein, the < /b> amino-acid sequence of < /b> the < /b> protein wasdetermined using a peptide sequencer. The < /b> peptides of < /b> the< /b> 105-kDa ... proteins [4]. HSP90contains two independent chaperone sites both in the< /b> N-terminal and C-terminal of < /b> the < /b> protein [5,6]. Eachchaperone activity of < /b> the < /b> protein will be inhibited bydifferent antineoplastic...
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Từ khóa: calculate the molar mass and molecular formula of the solida step towards the molecular detection of life on marstruyện kinh dị allies of the nighttruyện allies of the nightebook allies of the nighttruyện kinh dị hunters of the duskNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM