... Santimone, M. (1996) The mechanism of porcine3878 N. Oudjeriouat et al. (Eur. J. Biochem. 270) Ó FEBS 2003 On the mechanism of a -amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes Naăma ... Thus the longer the substrate, the higher was the activity. Inhibition by acarbose Inhibition of amylose hydrolysis occurred in the presence of 1080 lM acarbose and the association constants ... equation v is the initial velocity, [E]0 the enzymeconcentration, [S] the substrate concentration, [I] the inhibitor concentration, Km the Michaelis constant and K1i, K2i, L1i, L2ithe...