... Overproduction and puri®cation of SulA fusion proteinin Escherichia coli and i ts degradation by Lon protease in v it ro .Appl. Microbiol. Biotechnol. 43, 304±309.13. Higashitani, A., Ishii, Y., Kato, ... (1985) Insertionalmutagenesis of the lon gene in Escherichia coli: lon is dispen sab le .J. Bacteriol. 164, 1124±1135.5. Maurizi, M.R. (1987) Degradation in vitro of Bacteriophage kNprotein b ... polypeptide chain except for Leu158-Ser159,which is in the C-terminal region. The central region wasreported to be important for t he activity of SulA as a cell-division inhibitor, including essential...