báo cáo khoa học: " Position of the Phi and Po2 loci in the Hal linkage group in pigs" ppsx

Báo cáo khoa học: Roles of adenine anchoring and ion pairing at the coenzyme B12-binding site in diol dehydratase catalysis pptx

... reaction(s).Fate of the adenosyl group of AdoCbl in inactivation of a mutant holoenzyme duringcatalysisTo study the fate of the adenosyl group, the upperaxial ligand of AdoCbl, in the inactivation of the Sa224A ... bonding of N3with the side chain OH group of Sera224, and of 6-NH2, N1 and N7 withmain chain amide groups of other residues. A salt bridge is formedbetween the e-NH2 group of Lysb135 and the ... that the appro-priate hydrogen bonding between the side chain OH group of Sera224 and N3 of the adenine ring of the coenzyme adenosyl group is important for activation of the Co–C bond in the...

Báo cáo khoa học: Effects of sphingomyelin, cholesterol and zinc ions on the binding, insertion and aggregation of the amyloid Ab1)40 peptide in solid-supported lipid bilayers ppt

... domain tobe a consequence of lipid mixing within these microdomains, resulting in the presence of small amounts of SM and cholesterol in the DOPC micro-domain. When 1 mm zinc was present, an increase ... binding, resulting in shifts to larger angles (inset to B) and binding at higher con-centrations resulting in shifts to smaller angles. Binding to the SM-rich domain is shown in (B) and to the ... spectroscopy to follow the effects of sphingomyelin, cholesterol and zinc ions on the binding and aggregation of the amyloid b peptide1)40 in lipid bilayers. With a dioleoyl-phosphatidylcholine (DOPC)...

Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx

... supple-mented in culture media on the in vitro development of bovine embryos, and to examine the effects of glucose,phosphate and citrate upon the developmental frequency of bovine embryos.Materials and ... 3. Effects of glucose and/ or phosphate in the tw o-step culture system The effects of glucose and/ or phosphate were examined in Effects of Protein Source and Energy Substrates on the In Vitro Development ... 1320-1325.12.Lee, E. S. and Fukui Y.Synergistic effect of alanine and glycine on bovine embryos cultured in a chemicallydefined medium and amino acid uptake by in vitroproduced bovine morulae and blastocysts....

Báo cáo khoa học: " Effects of endomycorrhizal development and light regimes on the growth of Dicorynia guianensis Amshoff seedlings" pptx

... (HLI) and the values of the soil temperature in the pots ranged from 42 to47 °C. The soil temperature recorded under the two othertunnels (i.e. 1% and 14% of full sunlight) was in the range of ... October 1997, the leaflets of the seedlings were counted every 8–12 days and the height of their stem measured from the soil levelto the apical meristem, in order to describe the kinetic of leaf production ... (HgCl2) for 5 min and rinsed four timeswith sterile water. The seeds were then kept in asepticconditions during the germination phase. The rootemerged within one week, and the germinations weretransplanted...

Báo cáo khoa học: "Effect of article genotype and cutting type on the vegetative propagation of the pine hybrid (Pinus brutia (Ten) x Pinus halepensis (Mill)" pptx

... exists withinbuds between uxin and endogenouscytokinin, which on several occasions results in the dominance of the apical bud (higherauxin level). Growth of the inhibited ... regardless of the cutting type and hormone level. Severalother workers have also noticed the effect of genotype on rooting of conifer cuttings(Thulin and Faulds, 1968; Kleinschmit ... of terminal buds. Kum-merow and Hoffmann in 1963, reported thatkinetin could stimulate the growth of fasciclebuds. Other workers later reported thatremoval of terminal...

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... Ets-1 and Egr-1 are substrates of MAPK [12,43,44], the inhibition of MAPK by the incorporation of n-3 PUFAs could alter the phosphorylation of these transcription factors, affectingtheir binding ... doi:10.1111/j.1432-1033.2004.04385.xdetermine whether the incorporation of PUFA also affected the concentration of the p42/44 MAPK in caveolae.Pretreatment with EPA or DHA increased the amount of ERK1/2 protein, whereas the incorporation ... signalling mol-ecules [ 45–47]. Caveolin-3 is the main isoform in musclecells, including SMC [30]. Caveolin-1 and caveolin-3 seem toact as scaffolding proteins that negatively regulate the activity...

Báo cáo khoa học: "Responses of growth, nitrogen and carbon partitioning to elevated atmospheric CO concentration in live oak 2 (Quercus virginiana Mill.) seedlings in relation to nutrient supply Roberto" pot

... min after the fans were turned off in the evenings in order to maintainhigher concentrations in the chambers. [CO2] was mea-sured continuously in both the ambient and ... cm3) and maintained in the growth cham-ber. The tubes were filled with a mixture (v/v) of 90 %sand and 10 % peat; a layer of stones was placed in the base of each ... shown). In all treatments, the proportion of foliage (and roots) biomass declined (orremained constant) and stem biomass increased during the course of the experiment. The N...

Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... Least squaressuperposition of the P-loop main chain (starting at the residue prior to the catalytic cysteine and ending after the conserved arginine) resulted in rmsd values of < 0.75 A˚(supplementary ... upon substratebinding, due to the presence of a small b-barrel domainthat is unique to this protein and is involved in substrate binding [7,8]. No other residues in the proteinwere found to ... [18]. Treatment of PhyAsr with100 lm H2O2resulted in 33% of the enzyme beinginactivated after 10 min of treatment and 65% beinginactivated after 30 min. Incubation of the enzyme forup...

Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

... mm atropine, and 10)4m withoutatropine in the presence of 1 mm carbamylcholine,which induced the maximum increase in GTPcS bind-ing (Fig. 3B), suggesting that carbamylcholine caused the decrease ... over the detection sensitivity in the western blot analysis and well transferred in the membrane fraction.Binding properties of muscarinic ligands withM2::GOA-1 fusion protein The binding ... increase of GTPcS binding was completely inhibited byFig. 2. Binding of muscarinic ligands. Allexperiments were performed in triplicate.Each data point represents the mean ± SEM.(A) Binding...

Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx

... the kinetic constants forhydrolysis and synthesis of penicillin G, ampicillin and cephalexin. Furthermore, insight into the nature of the transition state in the rate-determining steps of the ... leaving group, p-nitrophenol, occupies the acyl binding site and the acetyl group binds at the leaving group binding site,sterically hindering the deacylating H2O molecule forefﬁcient nucleophilic ... binding of the nucleophile to the acyl-enzyme and displacement of the hydrolytic water molecule are the two most importantfactors in determining the reactivity of the nucleophile. The fact that the...

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