... deacetylcephalosporin C; DACS, deacetylcephalosporin C synthase; DAOC, deacetoxycephalosporin C; DAOCS,deacetoxycephalosporin C synthase; Dnase 1, deoxyribonuclease 1; EDTA, ethylenediaminetetraacetic ... bridgewith the carboxyl group of the a-aminoadipoyl- side-chainof penicillin N (1), with arginines 74 and 75 apparentlyplaying a less important role in side-chain binding. Takentogether, the results ... 2 )in Streptomyces clavuligerus [1–7]. The subsequent hydroxyla-tion of DAOC to give deacetylcephalosporin C (DAC 3)iscatalysed by a closely related oxygenase, deacetylcephalo-sporin C synthase...