... 94°C for 5 min, followed by 35 cycles at 94°C for 1min, 62-67°C for 1 min and 72°C for 1 min, followed bya final incubation at 72°C for 5 min.Screening of the TILLING libraryAmplicons analyzed ... kernels was deter-mined by Megazymes Total Starch Assay Kit (AA/AMG, Megazyme Pty Ltd., Wicklow, Ireland).AcknowledgementsThis study is partially financially supported by the European Commission ... M, Yamamoto Y, Satoh H, Shimamoto K: Nonsense-mediated decayof mutant waxy mRNA in rice. Plant Physiol 2001, 125:1388-1395.50. Miura H, Sugawara A: Dosage effect of Waxy genes on amylose synthesisin...