... locus
in the asexual pathogenic yeast Candida albicans. Science
1999, 285:1271-1275.
10. Hull CM, Raisner RM, Johnson AD: Evidence for mating of the
‘’asexual’’ yeast Candida albicans in a mammalian ... fungi)?
C. albicans has rarely been isolated in nature away from an
animal host and has probably co-evolved along with humans
for millions of years. It is presumed, th...
... the innate
immune system in the regulation of the antibody-mediated
inflammatory response. In addition, we have shown here
that already activated CII-reactive T cells reactive to glyco-
sylated ... study.
Monoclonal antibodies were generated as culture superna-
tants and purified by affinity chromatography with γ-bind
plus affinity gel matrix (Pharmacia, Uppsala, Sweden). The
Ig...
... to a chain of care structure in
the CHS in the hospital's catchment area. Maintaining a
structured collaboration with aftercare providers and
having a team or a coordinator in the hospital ... participated
in the acquisition of data. All authors participated in the interpretation of data,
revised it critically for important intellectual content and hav...
... with C. albicans in the presence of laminarin (Figure
6). Laminarin had no effect on levels of synovial fibroblast
COX-2 mRNA in the absence of C. albicans. Infection of syn-
ovial fibroblasts with ... by macrophages in
response to zymosan and C. albicans infection [43]. In the cur-
rent study laminarin partially blocked the increase in COX-2
mRNA that is seen...
... during the
course of our work was the ability to scale beyond 50 M reads,
which enabled the direct integration of Illumina PE read data
in a single Forge assembly stage. The increased accuracy of
EST-to -genome ... such as reads with mate pairs
anchored in a scaffold, polymorphisms within a repeat family,
or the combination of a low probability overlap and read-...
... pregnancy can cause a
delay in the diagnosis and appropriate treatment. The
first laparoscopic surgery during pregnancy was
cholecystectomy, performed in 1991
[14]. Thereafter,
laparoscopy ... dilatation and curettage, was admitted to the emergency
department because of sudden severe abdominal pain; the gestational age as calculated
by sonography was 14 weeks. Diagnost...
... DISCUSSION
The main aim of this study was to analyse the cold shock
response in S. pombe,ayeastthatdisplaysacascadeof
stress activated protein kinases homologous to the SAPK
pathway present in higher ... conditions.
The identification of a highly conserved SAPK pathway
in the fission yeast Schizosaccharomyces pombe allows to
analyse the precise mechanisms by which SAPKs...
... primers:
AACTTCAAA
CAGTTTGAGCAG for EF -A- mutation,
GACTTTCAA
CAGTTTCTCAAC for EF-B-mutation, GAT
TACACA
CAGTTCGAGGCA for EF-C-mutation, AATTT
CCAG
CAGTTCATGAAC for EF-D-mutation. The under-
lined ... may be the
case that the mutation from glutamate to glutamine in the
mutants induced a similar EF-hand conformational change
that occurs on the binding of Ca
21
in th...
... ectopically expressed cdk3 in
association with either cyclin E or cyclin A by transient
transfection (Fig. 2A) . Kinase assays indicated that pRb-
phosphorylating activity was prominently upregulated ... phosphorylated by cyclin A/ cdk2, cyclin E/cdk2,
cyclin A/ cdk3 or cyclin E/cdk3 which is produced in the
baculoviral system
We initially tested a hypothesis that p70
ik3-1
might...