... whether the disruption of cell–cell adhesion by Decma influenced expression of the uPA gene, weexamined change in uPA mRNA levels. Northernblot analysis showed only barely detectable levels of uPA ... enhanced expression of the uPA gene and, to alesser extent, of the MMP-9 gene (data not shown).Disruption of cell–cell adhesion by calcium chelationusing ethylene glycol bis (b-aminoethylether)-N,N,N¢,N¢-tetraacetic ... of allShc isoforms strongly reduced Decma-induced Erk phos-phorylation. Moreover, expression of silent mutants of Shc isoforms showed that only p46Shcand p52Shcres-cued the effect of the...