... transfer during the stoichiometric
reduction of CPR with NADPH.
Summary of results
Scheme 2 outlines possible reasons for the diminishing
intermediate formation of blue di-sq at increasing pH,
when ... residue (Trp677 in rat,
Trp676 in human CPR) stacks against the isoalloxa-
zine ring of the FAD cofactor thereby preventing
hydride transfer from NADPH to the flavin-N5 and
thus...
... glycine for 15 min at
room temperature and then with a solution of 50 mm
NH
4
Cl in NaCl ⁄ P
i
. Slides were washed with NaCl ⁄ P
i
and
incubated in NaCl ⁄ P
i
containing 5% BSA for 30 min. For
immunostaining, ... content of macrophages was increased by overexpression of
adipophilin and ⁄ or loading with VLDL. In contrast, inhibition of adipo-
philin expression using...
... reorientation of domains I and II. Thus, a model of
the closed form of TOP was created by separately fit-
ting domains I and II of the open TOP crystal struc-
ture onto the structure of DcP in its closed form ... TOP domains superimposed very well on the DcP
structure, with rms deviations of 1.50 and 1.21 A
˚
for
domains I and II, respectively. After fitting a...
... occur during the initial 6 h window of infection.
We infected RPE cells with K26GFP [27], and stained
cells for F-actin, using phalloidin at 30 min and 6 h
postinfection, and examined the cells ... points and
stained for F-actin (red). The GFP intensity (represent-
ing virus production) increased significantly over time,
as seen pictorially in Fig. 3A–E and in graphical for...
... that five of the 11
cysteinyl sulfurs act as bridging ligands between two
metal centers, and the remaining six act as terminal
ligands by coordinating to a single metal center. The
numbering of the ... mechanisms of the individual domains, in the
hope, initially, of simplifying the interpretation of
the metallation details of the two-domain protein. The
results presented here a...
... huntingtin, parkin and prion [22,24,25,28],
results in aggresome formation and impairment of the UPS
and, in some cases, apoptosis [24,28]. For example, expres-
sion of polyglutamine-expanded ... doi:10.1046/j.1432-1033.2003.03722.x
degradation of multi-ubiquitinated target proteins (i.e. cell
cycle regulatory proteins, such as cyclins and cyclin-
dependent kinase inhibitor...
...
entropy models for information extraction. In
Proceedings of ACL-2004
Xiao-Li Li and Bing Liu. 2003. Learning to classify
text using positive and unlabeled data. In
Proceedings of IJCAI-2003. ... Mining Inter-Entity Semantic
Relations Using Improved Transductive Learning.
In Proceedings of ICJNLP-2005.
Shubin Zhao and Ralph Grishman, 2005. Extracting
Relations with...
... Medical Institute,
University of Washington, Seattle, WA, USA
Understanding the sequence determinants of protein struc-
ture, stability and folding is critical for understanding how
natural proteins ... proteins [49]. In this system the queried
protein is inserted into a loop of another protein (host
protein). The basis for the selection is the ability of the host
protein to...
... research envir~t. Inplementatiorm
are ofte~ in an envirormm~t which requires some in-
depth ~mderstanding and support of LISP systems. Re-
cently much of
the
infornmtion on
the ATN
formalism, ... reordered by specifying the starting
state for the arcs of inCerast using the 0 command. ~e
user is then requested ~o specify
the r~
ordering of ~Se
arcs.
Insertion and...