... °C for 2 min, followedby 10 cycles of 94 °C for 20 s, 62–65 °Cfor30s ,and6 8°C for 3 min, followed by 12 cycles of 94 °C for 20 s, 62–65 °C for 30 s, and 68 °C for 3 min + 20 s increment per cycle,followedbya20-minholdat68°C. ... outerends of the full sequence and used to amplify a fragment of approximately 3500 bp from caterpillar head first-strandcDNA. To ensure the accuracy of the sequence data, thisamplification was performed ... pharmacological profile similar to that of other invertebrate DATs but distinct from that of mammalian DATs. The enigmatic pharmacological profile of TrnOAT does not allow it to fit easily into this model. Itis...