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Báo cáo khoa hoc:"Mapping the Naked Neck (NA) and Polydactyly (PO) mutants of the chicken with microsatellite molecular markers" docx

Báo cáo khoa hoc:

Báo cáo khoa hoc:"Mapping the Naked Neck (NA) and Polydactyly (PO) mutants of the chicken with microsatellite molecular markers" docx

... genetic map of the chicken, and open the way to the identification of the molecular nature of two develop-mental mutations of the chicken that are known to occur in many breeds of chickens. chicken ... mol´eculaireMapping naked neck and polydactyly in chicken 79Table I. Distribution of phenotypes and corresponding genotypes in the progeny of amale, double heterozygous for the naked neck ∗NA and polydactyly ∗PO ... assigned to the linkage group IV of the “classical” map [20]. The naked neck mutation is characterised by a reduction of feathered areas,mainly of the neck, but also in other regions such as the ventral...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... minimal.Morphological evidence of the antiapoptoticeffect of HSSAs shown in Fig. 2A,B, the treatment of cells with 50 lm CCCP induced profound changes in the nuclearmorphology of hepatoma cells, with chromatin ... opening of the nonselective, highly conductive PTP in the mitochon-drial IM [39]. The exact molecular composition of the PTP remains unclear. When MPT occurs, MPcollapses, leading to the failure of ... cellstransfected with the HSS siRNA as compared with controls (Fig. 7C). This indicates that there was animpairment of the permeabilization transition pore(PTP) of the mitochondrial membrane and that...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... 6-branch, and believed to befrom a contaminant (see below), having the same molecular mass with one Fuc in each of the 3- and 6-branches.Comparison of the product-ion spectra of the singly and doubly ... Leadeterminant.As the C3aion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position of the second Fuc 3-linked to the internal ... downfield from the Fuc IX H5 proton. ForTFiLNO, the same temperature would have resulted in totaloverlap of the H2O signal with the Fuc X and XI H5protons. Therefore the temperature of 27 °C was...
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Báo cáo khoa học:

Báo cáo khoa học: "Mapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic Parsing" docx

... behaviour of the verbs,which are not encoded in the SEM-I labelling and 191also some contradictory annotations in the mappingbetween PropBank and VerbNet. Furthermore, the use of the WordNet ... for all Sentences and Parsings which include the current verbdo3: Initialize vertices of the network with SEM-I labels and fea-tures.4: Initialize optionally vertices with the corresponding ... 3.2 Inference of semantic roles with BayesianNetworks The inference of semantic roles is based on train-ing of BNs by presenting instances of the featuresextracted, during the learning process....
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Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... inexploring the regulatory behavior of this enzyme. Toavoid misinterpretation of the results, the composition and stoichiometry of the bound cofactor(s) and the stability of the dimeric structure of ... The recovery of mono-meric epimerase from the spin column was 95% in terms of activity and 60% in terms of mass calculated on the basis of the dimeric enzyme [11].Models of inhibitor-free and ... epimerase. Abolition of the lag incatalysis of the native epimerase after interaction with these inhibitors was correlated with their inhibitor con-stants [23]. The values of the residual lag,...
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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot

Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot

... Thus, the lipoate residues of the complex mediate the regulation of its first component by the last product of the overall reaction, NADH.Reduction of the lipoate residues of the complexes in the ... these domains. Rather, the advantageappears to depend on the ability of the lipoyl group toprotrude from the inner core of the complex, indicative of the biological significance of the complex-bound ... (2-oxo acid, CoA and NAD+) and thiol-disulfidestatus of the medium. Accumulation of the dihydrolipoateintermediate causes inactivation of the first enzyme of the complexes. With the mammalian...
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Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx

Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx

... Encouraged by the results obtained, and to investigate the mechanism of macrocyclization, the steric demand of the C-terminal amino acid wasfurther increased by the incorporation of l-Met4(TL4) ... suggested that the size of the resulting ring and the preorganization of the substratedetermine whether a ligation or a cyclization step iscarried out [39]. Unfortunately, the prefold of the lin-ear ... the gene cluster enablingthiocoraline biosynthesis, could carry out the onlinemodification of the linear T-bound octapeptidein trans [27]. To further demonstrate that the stericdemand of the...
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Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt

Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt

... obtain the newstrains reported in Table 1.Genomic HA-tagging of SDH1Three consecutive copies of the HA epitope were fused to the 3¢-end of the SDH1 ORF in the genome of both the WT and EBY167-G418Sstrains, ... h of growth,each band represented about 30% of the total Sdh1-HA proteins. In the presence of FCCP (Fig. 4A,lane 2), a 60% reduction of the total amount of Sdh1-HA proteins was observed, the ... compartment. After 5 h of growth, the intensity of the 70 kDa band increased two-fold, and thisincrease was prevented by FCCP (Fig. 4A, lane 4).After 24 h of growth, the abundance of the 70 kDaform...
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Báo cáo khoa học: NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon ¨ Methanosarcina mazei Go1 pot

Báo cáo khoa học: NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon ¨ Methanosarcina mazei Go1 pot

... 0.25 and 0.5 lg of purified (His)6–NrpRII (lower panel) and 0.125, 0.25 and 0.5 lg of purified MBP–NrpRI (upper panel); elution in the absence of 2-oxoglutarate (lane 1), and in the presence of ... further verifying formation of a complexbetween NrpRI and NrpRII. The presence of 10 mm2-oxoglutarate significantly decreased the binding of MBP–NrpRI to the operator as well as the binding of the ... lanes 1 and 2, elution fractions in the presence of 250 mM NaCl; lanes 3 and 4, elution fractions in the presence of 500 mM NaCl; lanes 5 and 6, elution fractions in the presence of 1 M NaCl.The...
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Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx

Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx

... 110 and 182, and no tryptophans. Therefore, the shape of the near-UVCD spectra of EspA suggests the presence of some ter-tiary contacts around at least one of the tyrosines bothat pH 7.0 and ... cells[16].Although there is information available concerning the roles and structural properties of EspA filaments,to date, the conformation and the thermodynamicproperties of EspA have not been ... However, the presence of some aggregatedspecies was obvious from the FT-IR spectrum as discussedin the text.AcknowledgementsWe thank Prof Yuji Goto for the use of the CD spec-trometer and Miyo...
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