... obtain the newstrains reported in Table 1.Genomic HA-tagging of SDH1Three consecutive copies of the HA epitope were fused to the 3¢-end of the SDH1 ORF in the genome of both the WT and EBY167-G418Sstrains, ... h of growth,each band represented about 30% of the total Sdh1-HA proteins. In the presence of FCCP (Fig. 4A,lane 2), a 60% reduction of the total amount of Sdh1-HA proteins was observed, the ... compartment. After 5 h of growth, the intensity of the 70 kDa band increased two-fold, and thisincrease was prevented by FCCP (Fig. 4A, lane 4).After 24 h of growth, the abundance of the 70 kDaform...