Báo cáo khoa hoc:"The 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S-28S rDNA" doc

... 1626-1632.Original article The 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S-28S rDNA Ana InsuaRuth FreireJosefina ... time the nucleotide sequence of the whole 5S rDNA repeated unit of a bivalve species, the cockle Cerastoderma edule(Heterodonta, Cardiidae), and an analysis of the intra- ... of 5S rDNA at the telomere of the long arm. Afterperforming FISH with a heterologous 18S-28S rDNA probe and C-banding, absence of linkage between 5S and 18S-28S rDNA...

Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate speciﬁcity and are highly similar to proteins in Bacillus anthracis pot

... Allwater and acetate atoms were removed. Hydrogen atomswere added to the crystal structure, and the substrates and atomic partial charges were assigned to the protein and car-bohydrate atoms. Atomic ... N-terminus, and the second [H(X ⁄ P)DH] is located towards the middle of the protein. The crystal structure of BC1534 [10]revealed that the underlined H and D residues in the ﬁrst motif and the last ... organization and sequence alignments. Due to their high similarity to their B. anthracis homologs,clariﬁcation of their biological roles will contribute to a better understanding of the properties of...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... of pro-transforming growth fac-tor (TGF)-a [37], CD8a or the frizzled receptor Fz4[38], as well as in the Golgi retention of p24a, a mem-ber of the p24 family of cargo receptors [39].In the ... MT1-MMP ICD and GRASP55 and therefore its mutation to AAA573couldexplain the reduction of the level of cell surface expres-sion of this mutated version of MT1-MMP. It isimportant to note that ... MT1-MMP and GRASP55. Taken together, our observations suggestthat furin-mediated disruption of the MT1-MMPGRASP55 complex can lead to a reduction of the MT1-MMP, and a consequent decrease of proteaseactivity...

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... wasfound to be converted to a detectable extent. The methylgroup is probably transferred to tetrahydrofolate, the assumed second cofactor of the enzyme. Methylene-tetra-hydrofolate may then be ... but the covalent tethering of the flavin cofactor is anautocatalytic process [39] for which the active site has to be well defined [40]. The results of this work define a demethylating oxidase of novel ... be of general occurrence and hasbeen described in detail for different bacteria [31,33]. The similarity of the C-terminal domain of MABO to otherproteins of the sarcosine dehydrogen ase and...

Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc

... reduces the endocytic arm. This facilitatesuptake of zinc and restores normal cellular zinc homeo-stasis. On the other hand, when cellular zinc levelsare elevated, the rate of endocytosis of ZIP1 ... increase of cytoplasmic zinc concentra-tions by enhancement of zinc uptake or release of the stored zinc from subcellular compartments to the cyto-plasm of the cell when zinc is deﬁcient [18]. On the other ... USA). The DNA fragment in which the Myc epitope was fused in frame to the C-terminal end of ZIP1 was isolated from the resulting plasmid and cloned into the pcDNA5 ⁄ FRT vector (Invitrogen) to create...

Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx

... only 26%between the two enzymes. On the other hand the structureappears to be similar to that o f Pf LDH with a rmsd of 0.8between the Ca atoms (Fig. 5). Both the cofactor and the substrate binding ... due to the i nsertion of ﬁve residues into the loop inthecaseofPf LDH (Fig. 1). The substrate speciﬁcity loop of Pf MDH s hares a sequence identity of 65% with that of the MDH from C. tepidum and ... MDH and corresponded to the signature sequence GXGXXG, found in proteobacterial MDHs and LDHs, rather than the signature sequence of GXXGXXGfound in most of the MDHs. More d etailed analysis of substrate...

Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... FtGLDHAA and the artiﬁcial AtGLDH1AA,are indeed exclusively due to the insertion of twoadditional alanyl residues into the N-terminus of the H-protein.In conclusion, the substrate properties of the ... residuesvery close to its N-terminus, and is by far the mostdominant H-protein type in leaf mitochondria of theseplants. Because of photorespiratory metabolism, leafmitochondria contain much ... vglycerol and stored at )80 °C.MALDI-TOF MS The purity of recombinant proteins was assessed by SDS–PAGE [30], and the molecular identity of all H-proteinsincluding completeness of lipoylation...

Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt

... pAR[DRI] vector. Of the 750 000 plaques analyzed, two of them hybridized with the probe. The sequences of the inserted cDNA were identical.In frame with the kgt11 Lac Z coding sequence theycontained ... expressed anywhere other than the cytosol and the cytoplasmic surfaces of membranes [29]. On the other handaccurate su bcellular distribution of HIP/PAP had not beenstudied and thus it was considered ... hepatocytes and to bind elements of the extracellular matrix [8]. Moreover HIP/PAP has beenrecently reported to combine mitogenic and antiapoptoticfunctions regarding hepatocytes and to enhance...

Báo cáo khoa học: MBP-1 is efﬁciently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover doc

... speciﬁc to the 5¢-end of the 279- nucleotide sequence and to the 3¢-end of the ENO1cDNA was performed to clone out a 2552-bp full-length cDNA. Collectively, these data conﬁrm the exis-tence of ... (Stratagene, La Jolla, CA,USA), the PCR product was digested with BamHI and EcoRV and ligated into the pcDNA3.1 vector. The inser-tion of an HA-tag sequence into the 3¢ end of the ENO1ORF was performed ... primers (P1, P2 and P3)cloned out an additional 279 -nucleotide sequence upstream from the AL833741 sequence. This nucleotide sequence islisted at the bottom of panel A. The c-Myc and HIF1a binding...

Báo cáo khoa học: "Malignant gastrointestinal stromal tumor presenting with hemoperitoneum in puerperium: report of a case with review of the literature" ppt

... bleeding into the abdominal cavity.Figure 4 Demonstration of the resection of segmental bowel; the tumor was removed with a part of 3-cm of the smallintestine.Figure 5 The surgical specimen of the ... acase of recurrent metastatic GIST that failed extensivesurgical therapy and chemotherapy [21]. The dramaticresponse in their case was documented both clinically and histologically. They also documented ... revealed a largemass arising from the small intestine and growingexophytically out into the peritoneal cavity. On the top of the mass, distended vessels were observed and a ruptured superficialvessel...

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