... hormonemember of the insulin superfamily that includes nineother members in humans, including insulin, insulin-like growth factor-1 and -2, relaxin-1, -2 and -3, and INSL4, -5 and -6. INSL3 was cloned in ... eight-residue linker sequence. For insulin and insulin-like growth factor-1, the C-terminus of the B-chain and N-terminus of the A-chain can belinked by an extremely short peptide (0–2 residues) and the ... and nucleotide sequence of the recombinant human INSL3 precursor. The B-chain and A-chain are shown in red and green, respectively. The N-terminal 6· His tag and the linker between the B-chain...