... 86: 841-5. 13. Nakayama T, Soma M, Rahmutula D, Ozawa Y, Kanmatsuse K. Isolation of the 5'-flanking region of genes by thermal asymmet-ric interlaced polymerase chain reaction. Med Sci ... and Ms. H. Tobe, M. Nakamura, and K. Sugama for their technical as-sistance. This work was supported financially by a grant from the Ministry of Education, Science and Culture of Japan (High- Tech ... history of hypertension; all had an SBP less than 130 mmHg and a DBP less than 85 mmHg. A family history of hypertension was defined as having grandparents, uncles, aunts, parents, or siblings...