... penicillin binding by DAOCS [1, 12] a key featureof which is that arginines 16 0 and 16 2 bind to the carbonyl and/ or carboxylate of the bicyclic b-lactam nucleus, withthe pro-S methyl group projecting ... role of arginine residues in substrate binding and catalysisby deacetoxycephalosporin C synthaseSarah J. Lipscomb 1, *, Hwei-Jen Lee 1, 2,*, Mridul Mukherji 1 , Jack E. Baldwin 1 , Christopher ... syn-thase [20, 21] ) have suggested that prime substrate bindingactivates the enzyme–iron(II)-2-oxoglutarate ternary com-plex to oxygen binding, thereby initiating catalysis and ensuring coupling of...