... con-taining 10 μg/ml pepstatin/aprotinin/leupeptin, 1 mMsodium orthovanadate and 5 mM NaF. After removal of insoluble material by centrifugation at 10.000 × g and 4°C for 15 min, the protein concentration ... induced by treatmentwith human recombinant tumor necrosis factor and determined by flow cytometric measurement of propidium iodide uptake as well as by optical analysis of cell morphology. Results: ... mechanisms of ciPCD are just beginning to beunraveled, e. g. by the recent identification of RIP3,enzymes of the energy metabolism, Nox1, CYLD, Bmf, and cathepsin and calpain proteases as candidate...