Báo cáo khoa học: " Inhibition of homologous recombination repair with Pentoxifylline targets G2 cells generated by radiotherapy and induces major enhancements of the toxicity of cisplatin and melphalan given after irradiation" pot

... AccessReview Inhibition of homologous recombination repair with Pentoxifylline targets G2 cells generated by radiotherapy and induces major enhancements of the toxicity of cisplatin and melphalan given ... :Report of an IAEA meeting. Int J Radiat Oncol Biol Phys 2006,64:551-561.54. Bohm L: Inhibition of homologous recombination repair with pentoxifylline targets G2 cells generated by radiotherapy. Molecular ... fullycycling cells in the absence of Pentoxifylline. Addition of such low concentrations of common cytotoxins to G2 blocked cells in the presence of Pentoxifylline generatesvery large enhancements of...

Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

... werevisualized by autoradiography of dried gels. Speciﬁcity of binding to IRP-1 was demonstrated by competition with unlabeled ATP, ATP-cS, and unlabeled 8-azido-ATP, and by lack of competition with the ... activity of IRP-1 is also enhanced in the presence of IRE, we measured hydrolysis of [32P]ATP[cP] in the presence of 0–400 ng of IRE(Fig. 8). Compared to the amount of ATP hydrolyzed with 400 ng of ... dissociation of the com-plex. Enhancement of ATP hydrolysis by IRE in the presence of IRP-1, but absence of hydrolysis in the presence of IRE alone, suggests that ATP may interact with the complex....

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... respectively. The PDB code for the wild-type protein is2E4L. For the entire structure (A), N and C represent the N- and C-termini of the protein, and a and b represent the a helix and b strand,respectively. ... together with the distances. For the structure around residue 39 (C), the side-chains of Y24, residue 39, F41 and Q149 are shown. The hydrogen bondsbetween D39 and Od1 and Q149 and Ne, and between ... affect the protein structure, and areconsistent with the fact that the thermostabilizing effects of the mutationsare roughly additive. The introduction of favourable interactions and the elimination...

Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

... likely, the mature part of r pro-Der p 1 is notef®ciently substituted with 125I in the presence of the propeptide, in contrast with the recombinant matureDer p 1 after removal of the p ropeptide.In ... Both the lack of enzymatic activity and the hypoallergenic character makethis recombinant a potential safe candidate for a pplicationin allergen-speci®c immunotherapy. To further evaluate the potential ... 6B). The 25-kDa mature band w asrecognized by rabbit antibodies against nDer p 1, con®rm-ing the identity of the 25-kDa band as Der p 1 (not shown). The 10-kDa fragment referred t o as the propeptide...

Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx

... 3 h of chase, only 25% of trun-cated pFGE were retained within the cells (Fig. 4B). On the contrary, very little of the wild-type and the KDELform of pFGE was secreted during the pulse, and ... wereloaded at a ratio of 10 : 1. The amount of total pFGE in cells and medium and the percentage of intracellular pFGE are given below the lanes. Two differentially glycosylated forms of pFGE (arrows) ... (see ﬁgure legend). After 6 h of transfection, the cells were induced with 2 lgÆmL)1doxycycline. After 24 h of transfection, the cells were washed twice with NaCl ⁄ Pi,ﬁxed with 4% paraformaldehyde...

Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx

... inhibition of each of the tested compounds. These data were then compared with the experimentally determined data (percentage inhibition at 100 lm), and the results are presented inFig. 8. The ... that the presence of a nitro group at the 5-position was associ-ated with stronger inhibition (1c compared with 1d; 1h with 1i) and this was positively associated with the presence of the 3-methoxy ... constantstirring, and monitored by TLC. The reaction wasstopped by the addition of distilled water (50–100 mL) and the product was extracted into ether (3 · 40 mL). The ether extracts were...

Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... FEBS 373Thermal unfolding transition in the absence and in the presence of the different ligands The influence of ligand binding on the thermal stability of C-LytA was studied by monitoring the CD ... in the separ-ation of the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of ... induced by ligands The influence of ipratropium and atropine on the structure of C-LytA was first analyzed by near-UVCD, as the far-UV CD signal is not recordable, due to the high absorption of these...

Tài liệu Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide docx

... 2B). The addition of 20 lMZn-ferrocyanide in the reaction medium from the beginning of the measurement leads to the appearance of the reduced form of the enzyme, even in the presence of the ... together, these resultsmay support the idea that the ﬂavin itself may be involved in the interaction of the ﬂavoproteins with the metal. The kinetic analysis of all FNR activities inhibited by Zn-ferrocyanide ... between the extent of Zn2+ inhibition and nicotinamide ring o ccupancy in the FNR v ariants.These observations indicate that the binding of NADP+to the enzyme either reduces the accessibility of...

Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

... inactivation of G APDH being metal-ion independent, whereas inhibition of GR and LDH by H2O2occurs most rapidly in the presence of metal ions.It is proposed that the inhibition of GAPDH and GR by these1O2 -generated ... supported by the inefﬁcient inhibition of LDH by theseperoxides, in the absence of metal ions, as this enzyme doesnot contain an active site thiol. The nonradical nature of the GAPDH and GR inhibitio ... slow thermal i nactivation.Examination of the role of peroxide-derived radicalsin enzyme inhibition To examine whether radical species were generated during the inactivation of GAPDH and L DH by...

Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf

... tumor cells exhibiting IR; 1, < 25% of the tumor cells exhibiting IR;2, 25–50% of the tumor cells exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% of the tumor cells exhibiting ... characteristics and speciﬁc immunoreactivity (IR). The staining was semi-quantitatively evaluated by assigning a score for the inten-sity of the IR and for the proportion of cells positivelystained. The ... (Fig. 4A). Inhibition of the PI3K/Akt pathway decreased the expression of P-gpinduced by PrPCat both the mRNA and protein levels,suggesting transcriptional regulation of P-gp by PI3K/Akt...

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