... efficacy. Application of such auto-
mated radiogrammetry to the existing clinical trial data (such
as these hand X-rays) offers an opportunity to examine the
effects of TNF blockade on bone loss and ... technique of
metacarpal morphometry employed in these three old studies
is identical with that measured by modern day automated
radiogrammetry. Moreoever, automated radiogram...
... healthy donors. BM revised the article critically.
JLD was involved in the design of the study and reviewed the
article critically. AC was involved in the conception and design
of this study, in the ... E-selectin and intercellular adhesion
molecule, necessary for the flow of leucocytes into the sub-
endothelial tissue [6]. It also promotes production of other
pr...
... improved greatly the quality of life of the patients,
and was shown to significantly retard the structural damage
that is typical of this chronic inflammatory disorder. In the field
of spondyloarthropathies ... necrosis factor- α
(TNF-α) proved highly efficacious, with an acceptable safety
profile in the chronic treatment of rheumatoid arthritis. Not
only did the...
... closely the activation of MAPK by TNF in vivo, we
histologically assessed joints of hTNFtg mice and wild-type
mice for phosphorylated forms of p38MAPKα, ERK and JNK.
Synovial inflammatory tissue of ... effects of systemic
blockade of TNF, IL-1 and receptor activator of nuclear factor-
κB (RANK) ligand on the activation of MAPKs were assessed.
In vivo, overexpress...
... necrosis factor- α stimulates dehydroepiandrosterone
metabolism in human fibroblast-like synoviocytes: a role for
nuclear factor- κB and activator protein-1 in the regulation of
expression of cytochrome ... Bjorkhem I, Seckl J, Lathe R: Cyp7b, a novel brain cyto-
chrome P450, catalyzes the synthesis of neurosteroids
7alpha-hydroxy dehydroepiandrosterone and 7alpha-hydroxy...
... promotes further expression of
cytokines and chemokines by synovial cells and chondro-
cytes, thereby sustaining a renewal of local inflammatory
mediators (reviewed in [9,10]). The presence of TNF-α ... loss and changes in cell morphology. The
MAPK/ERK pathway is thereby at least partially involved in
regulating the aggrecan and type II collagen genes and in
remodeling...
... only
transitory, because a portion of YopD is also localized to the
host cell cytosol [10]. In addition, we and others observed
that a yopD null mutant is constitutively induced for
synthesis of ... spin system assignments of
YopD
278)300
were obtained using COSY and TOCSY
spectra and a NOESY spectrum was used to identify
sequential backbone connectivities. A comparison of...
... or
TBP, and taken as the loading controls, and also to assess
the purity of nuclear and cytosolic preparations. Bands
were analysed densitometrically as described below.
Immunofluoresence microscopy
HepG2 ... agarose gel, photographed
with the Alpha Innotech gel documentation system and the
intensity of each band was analysed densitometrically and
plotted after normaliz...
... was involved in the conception and
design of the study, analysis and interpretation of the data, and
did most of the drafting of the manuscript. All authors read and
approved the final manuscript.
Acknowledgements
We ... proteoglycan degradation in carti-
lage tissues can be mediated by both the production of NO
and the involvement of matrix-degrading enz...